| Oldenlandia diffusa(willd) Roxb is scoop out from the folks, having various physiology the medium herb medicine of the activity. Have the pure heat counteract poison,the benefit urine decrease swelling,live the function that the blood relieve pain. Clinical application extensively,the lord cures a malignant tumor,enterogastrtis, urine system disease,snake to bite etc. disease.This thesis includes two parts:The first part introduces the herbal medicine origin , chemical components , pharmacological effects , clinical applications and the recent development of application of fingerprint chromatogram.The second part can be divided into four aspects:1.Research the chemical components of Oldenlandia diffusa(willd) Roxb, extracted and separated some compounds and confirmed the structures through the method of UV, IR, MS, NMR.2.Determining Ferulic Acid and p-coumaril acid through UV spectrophotometry and HPLC chromatogram and establishing of the quality standard of Oldenlandia diffusa(willd) Roxb.3. Research the recent development of application of fingerprint chromatogram, and build up the fingerprint chromatogram of Oldenlandia diffusa(willd) Roxb on HPLC4.By GC-MS and spectra searching database, the constituents of the volatile constituents from the extracted fraction were identified first step. |