Curcumin Of As <sub> 2 </ Sub> O <sub> 3 </ Sub> Preliminary Study Of The Mutagenicity And Induction Of Apoptosis Of Tumor Cells,

Objective1 To research the effect that curcumine resisting the mutagenicity of As₂O₃ and the dosageeffect of which.2 To study the effect that curcumine combined with As₂O₃ restrain growth of the human hepatoma carcinoma cell HepG₂,the human gastric carcinoma cell SGC7901 and the human cervical carcinoma cell HeLa and induce apoptosis of cell HeLa.3 To approach the possible mechanism which curcumine resist mutation and tumors ,in order to develop health protection drug of anti-mutagenesis and provide experimental basis which curcumine combines with As₂O₃ to resist tumour in clinical application.Methods1 The research on curcumine resisting the mutagenicity of As₂O₃. â‘ The mouse bone marrow polychromatic erythrocyte micronucleus test. All groups use salad oil or different density curcumine solution by intragastric administration for 7 days.At the 7th day, all groups give physiologic saline or As₂O₃ by intraperitoneal injection.Colchicine be injected before putting mouse to death. Direct smear method to prepare the sample of micronuclei for every groups.To count 1000 polychromatic erythrocyte and record the amount of micronuclei. â‘¡The demic cultivated lymphocyte in vitro micronucleus test.The human peripheral blood lymphocyte be cultivated by aseptic technique approach for 24 hours.All groups be given different density As₂O₃ solution or curcumine solution . 44 hours later ,colchicine be injected.Then 4 hours later,airing method prepare the sample of micronuclei for every groups.Then cells be counted under light microscope by double blind method.To count 1000 lymphocyte cells and record the amount of micronuclei.2 The research that curcumine combined with As₂O₃ induce apoptosis of these tumour cells. â‘ Curcumine and As₂O₃ effect cell SGC7901 and cell HepG₂ on the growth and apoptosis. Cells in log phase growth be cultivated in 96 holes plate.24 hours later, all groups give serum-free medium,different density As₂O₃ solution or curcumine solution .Afer 24 hours , the growth state of cells be observed in each group.Then optical density (OD) of each hole be detected by MTT method,then calculating the growth inhibition ratio.â‘¡Curcumine effect cell HeLa on the ratio of apoptosis and cell cycle. Cells in log phase growth be cultivated in 24 holes plate. 24 hours later, all groups give serum-free medium,different density As₂O₃ solution or curcumine solution .Afer 24 hours , the growth state of cells be observed in each group.To use flow cytometry (FCM) to detect that curcumine effect cell cycle and apoptosis of cell HeLa by PI simple staining method, and count the raito of apoptosis. SPSS statistics software deal with the data.Results1 The mouse bone marrow polychromatic erythrocyte micronucleus test.The raito ofmicronucleu is the greatest in As₂O₃ group.The raito of micronucleu is the lowest in As₂O₃ +middle desity curcumine group .Comparing with the vacuo contrast group , As₂O₃ + high,middle, low desity curcumine group have significant difference (p<0.05) ;As₂O₃ group andAs₂O₃ + high desity curcumine group have more significant difference (p<0.01).2The demic cultivated lymphocyte in vitro micronucleus test.Accompanied with increasing of...