We investigated constituent of steroidal saponin of the fruits of Tribulus terrestris L.,5compounds were isolated from it by using open-column(silica gel, macroreticular resin), preparative HPLC methods et al. On the basis of spectroscopic analysis including 1HNMR, 13CNMR, ESI/MS,1H-1HCOSY, HMBCand chemical methods, the structure of 5 compound were identified As ( I ) 25R-5 α -spirostan-12-one-3β -ol -3-O- β -D-glucopyranosyl (1â†' 2 ) - β -D-glucopyranosyl- ( 1 â†' 4 ) - β -D- galactopyranoside ; ( II ) 25S-5 α --spirostan-12-one -3 β -ol -3-O- β -D-glucopyranosyl(1â†'4)- β -D- galactopyranoside; (III) 25R-5 α - -spirostan-12-one-3 β -ol -3-O- P -D-glucopyranosyl(1â†'2)-[ P -D- xylopyranosy(1â†'3)]- β -D-glucopyranosyl(1 â†' 4)- β -D -galactopyranoside ; ( IV ) 26-O- β -D-glucopyranosyl - (25R) -5 α - furost -3 β -22 α , 26-triol -3-O-[ β -D- xylopyranosyl(1 â†'3)]-[ β -D- xylopyranosyl(1 â†'2)] β -D-glucopyranosyl(1â†'4)-[ α -L-rhamnopyranosyl(1 â†'2)]- β -D -galactopyranoside; ( V ) 26-O- β -D- glucopyranosyl -22-methoxy- (25R) -5 α - furost -3 β , 26-diol -3-O-[ β -D- xylopyranosyl(1 â†' 3)]- [ β -D- xylopyranosyl(1 â†' 2)] β -D-glucopyranosyl(1â†'4)-[ α -L-rhamnopyranosyl(1â†'2)]- β -D -galactopyranoside。This topic I has carried on the pharmacology experiment to the compound I , finally indicated: The compound I compares with the aspirin in to the mouse hemoglutination time influence aspect, the compound I may extremely obvious lengthen the hemoglutination time, meanwhile can obviously lengthen the time which the mouse in vivo thrombus forms.This topic has provided the science rationale for the puncture vine pharmacodynamics function and the action mechanism research of the fruits of Tribulus terrestris L.and for the puncture vine innovation medicine development. |