Studies On Constituents, Biological Activities In The Tribulus Terrestris L. Fruit

In this research, the chemical composition of Tribulus terrestris L. fruit has been systematically studied, and the pharmacological effects of related compounds was also studied. Seven compounds were isolated from the fruit of Tribulus terrestris L. on column chromatography of silica gel, Sephadex LH-20, and ODS. These compounds were identified on the basis of chemical and spectral methods as:3-O-β-lycotetraosyl 3β-hydroxy-5α-pregn-16-en-20-one (Ⅰ),3β-hydroxy-5α-pregn-16(17) en-20-one-3-O-β-D-xylopyranosyl(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-galactopyr anoside (Ⅱ), terrestriamide (Ⅲ), daucosterol (Ⅳ), hecogenin-3-O-β-D-galactopyranoside (1-2)-[β-D-xylopyranosyl (1-3)]-β-D-glucopyranosy (1-4)-β-D-galactopyranoside(Ⅴ), benzoic acid(Ⅵ) tigogenin-3-O-β-D-galactopyranoside-[(1-2)-α-L-rhamnose]-(1-4)-β-D-glucopyranosy-[(1-3)]-β-D-xylopyranosyl, (Ⅸ). CompoundⅠwas isolated from the family Zygophyllaceae for the first time, and compoundⅡwas firstly reported from Tribulus terrestris L.We found that D101was more suitable than AB-8 on the separation of Tribulus terrestris L.50% ethanol part contains more saponins,which separation from polar water-soluble components and smaller soluble components.Antioxidation bioactivity of four compounds(Ⅰ,Ⅱ,Ⅴ,Ⅸ) isolated from the tribulus terrestris L. fruit was tested. On the basis of experiment data, the link of structure and activity was discussed. The inhibitory activity of steroidal saponins better than the C21 steroidal saponins. We also studied the effect of compoundⅤand extraction layers on auricula edema in mice by induced dimethylbenzene. CompoundⅤshowed inhibit activity.