Neonatal Rat Cranial Suture The Role Of Early In Vitro Stretch Of No And Nos

Part 1In vitro calvaria culture as a model for tensile stress studyon cranial suture in neonatal ratsObjective: To establish an in vitro calvaria culture model in neonatal SDrats. And to discuss the possibility and effect of the model under tensilestress .Methods: Calvaria samples with middle cranial suture (10.0 x 10.0 mm~2)were achieved from one week old Sprague-Dawley rats. Helical springswere put on both experimental and control groups. The springs werecalibrated to deliver 0.2g (0.00196N) of tensile force on the experimentalgroup with no force on the control group . After 0h, 1h, 6h, 24h, 48h ofincubation, the specimen were collected for histological examination.Results: Compared with the control group, the migration andproliferation of osteoblasts and fibroblasts as well as the angiogenesis ofblood capillaries were significantly increased in the experimentalgroup, suggesting the active growing status in the cranial suture region.New bone was deposited on the bone surface obviously. No baterialinfection or tissue damage was observed.Conclusion: Tensile stress has an effect of expanding cranial sutures. Thecranial suture can live and develop well in vitro calvaria culture.