| Objective: To investigate the prevalence of Cervical cancer and Cervical intraepithelial neoplasia(CIN) of women in high sources city setting as well as positive rate of high risk human papillomaviruses (HPV) and distribution of some certain important types of HPV in different sub-population and provide base line data to future vaccine intervention on cervical cancer. To investigate the clinical value of one human papillomaviruses test method- Yaneng gene chips technology in cervical cancer screening.Methods: A population-based cervical cancer screening was done finally in 1137 women with age between 15~59 between 2004.11～2004.12. Subjects were residents, workers and servicing women from the overseas Chinese town of the Nanshan district, Shenzhen city and were stratified sampled according certain age groups. After consent and questionnaire performed, clinical doctors collected exfoliated cervical samples for liquid-based cytology test, both of HPV DNA hc2 test(13 high risk types) and Yaneng gene chips test (13 high risk HPV types,5 low risk types and 5 other types) were performed at the same time using the residual cervical cell samples. VIA, coloscopy examination, and biopsy was provided later if any abnormal lesion were found. Eventually, the golden standard to evaluate all screening method is pathology results. SPSS 10.0 was used to analysis the data withχ2 test, trendχ2 test, Logistic regress, Kappa test. Sensitivity, specificity, predictive value and something like that were calculated to valuate the screening methods.Results: Eligible 1137 women went through above examination procedure finally. There were no cervical cancer, 3 cases CIN III, 11 cases CIN II, 36 cases CIN I. HPV DNA (hc2) detection rate in the polulation was 14.0%. Double peaks were showed in age curve owing to the rates of two age groups(25~29 and 50~59)( 17.7%'21.0%). Rate of 35~39 group was the lowest (8.8%), but there are no significant between age groups. Rates of high risk HPV of community women, workers and servicing women were 14.1%,9.2% and 18.9% respectively. Two variables (the NO. of sexual partner in lifetime and source of population) have significant in the multi-factor model. The most popular types of high risk HPV were 16, 18 and 58 in positive women HPV and the percentages and them were 29.7%,18.9% and 18.9%in turn. The positive rates of different age group of low risk HPV were 1.4% (~34), 1.7% (35~44), 3.2% (45~)respectively. The rates of 5 low risk types and other types of gene chips test in this population were 1.9% and 0.2% by turn. Detection rate of gene chips test for common 13 kind of high risk was 9.8% there was significant differences(P=0.002) if compared with hc2 test. The two methods consistence was common (kappa=0.498). The sensitivity, specificity, accuracy, positive prevalue, negative prevalue, positive likelihood ratio and negative likelihood ratio of yaneng... |
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