Recover More Experimental Study Of The Impact Of Fatty Liver In Rats, The Liver Capsule

ObjectiveThe creation of FuYuan YuGan Capsule was guided with the theory of TCM and combined with the achievement of modern pharmacology. It had benefitical effect on fatty liver.In order to expound its pharmacological action and probe into its mechanism of action,the task had observed the prevention and cure function for FuYuan YuGan Capsule in the treatment of nonalcoholic fatty liver. It provided scientific basis for clilic and laid one foundation for the further research and exploitation of new drug. MethodsWe produced the model steatohepatistis rats by feeding rats with high fat diet. Sixty male SD rats were divided into six groups randomly: groups of rats fed with high, median and low doses of Capsule FuYuan YuGan, and a group of rats fed with DongBao GanTai, a model steatohepatistis group, and a group of rats with normal liver funetion as a control. Capsule FuYuan YuGan and DongBao GanTai were given to the model rats with fatty liver, serum activities of liver-associated enzymes, blood lipids, liver triglycerides, fasting blood glucose , fasting plasma insulin , homeostasis model assessment-insulin resistance insulin resistance index(HOMA-IR), and the liver histology of rats of all groups were assayed. Flow CytoMeter and immunohistochemistry technique were used to detect the apoptosis of hepatocytes and the expression of Bcl-2, Bax in hepatocytes respectively.ResultsCompared with the model group, the levels of serum activities of alanine aminotransferase (ALT),aspartate aminotransferase(AST), ,total cholest erol (TC), triglycerides (TG) and liver triglycerides in the Capsule FuYuan YuGan groups were significantly lower and were similar to those of the normal group, Fasting blood glucose, fasting plasma insulin and HOMA-IR showed an increasing tendency, but there was no significant diference of those indexes among the three groups.The rate of apoptosis cells in fatty liver model group was significantly higher than that in treatment groups and normal control group(P<0.05). and the rate of apoptosis cells in treatment groups were significantly lower than that in fatty liver model group (P<0. 05),The expression of Bcl-2 in treatment groups were significantly higher than that in fatty liver model group (P<0.05), and the expression of Bax in these groups was significantly lower than that in fatty liver model group (P<0. 05).There were a lot of lipid deposition in the pathologic section of 1 iver tissues from the model group, and generous fat accumutat ion was observed microscopically. Only slight fat accumutation was observed in liver tissue from Capsule FuYuan YuGan and DongBao GanTai treated groups. No lipid deposition was observed in the pathologic liver sections from the normal group.