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Activating And France, The Benefit On Liver Cell Proliferation And Cell Cycle

Posted on:2006-04-20Degree:MasterType:Thesis
Country:ChinaCandidate:Y H ChenFull Text:PDF
GTID:2204360155461109Subject:Basic Theory of TCM
Abstract/Summary:PDF Full Text Request
Purpose: By comparing the influence of Danshen Root injection and Milkvetch Root injection on cell proliferation and cell period of normal human being's liver cell strain L02, we are intend to further lay the experimental ground for the research on the TCM theory of liver storing the blood and provide creative ideas and methods for treating liver diseases in clinic.Method: By the administration of directly adding Danshen Root injection and Milkvetch Root injection respectively with different concentrations into culture solution, we take the MTT method to test the different influences of the two kinds of injections on cell proliferation of normal human being's liver cell strain L02 and the flowing type analysis instrument to identify the different influences of the two kinds of injections on cell period of normal human being's liver cell strain L02. Result: Firstly, compared to the blank group, Danshen Root injection obviously has positive effect to the proliferation of human being's liver cell strain L02 (it changes markedly when the drug concentration in blood plasma is in 4,8,16,32 times, P<0.05). In addition, with more using of the dosage, there is a certain quality-effect relationship. Moreover, in liver cell strain L02, cells of G1 phase are in decline while cells of S and G2 phases are in rise which compared to the raw group, means the cell period is much more in advance. Secondly, compared to blank group, Milkvetch...
Keywords/Search Tags:Anti-blood stasis therapy, Tonifying therapy, Human being's liver cell strain L02, Cell proliferation, Cell period
PDF Full Text Request
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