Nettle Of Extraction, Structural Analysis And Functional

In folk medicine nettle plants have been used for diuresis, allergies, arthritis and rheumatism. The pharmacological effects of nettles such as anti-inflammatory, analgestic effect and inhibition of benign prostatic hyperplasia (BPH) were studied, and active components are reported to be polysaccharides. However, their characteristic and structure are not further studied.Urtica.cannabina, roots collected from Guizhou province, extracted with hot water and ultrafilted removing the small molecules to obtain the crude polysaccharide. The optimization of polysaccharide extraction was studied on the orthogonal design. The content of polysaccharide was determined by colormetric assay. The resultant extracting process was easy, rational and stable. Pharmacological test indicated that the crude polysaccharide had inhibition on benign prostatic hyperplasia in castrative rat induced by testosterone propionate, but the toxicity is very low. The crude polysaccharide also has analgestic effect on aching mice induced by acetic acid .The crude polysaccharide was removed protein by Sevage method, and purified by DEAE-Cellulos, DEAE-Sephadex A-25 and Sephadex G-100 gel filtration chromatography successively. Five homogeneous polysaccharides were obtained. The purities and molecular weights of these polysaccharides were determined by conventional gel filtration chromatography. Physico-chemical properties and structure features were elucidated by chemical methods (acetylation, methylation analysis, etc) and spectral methods (IR, 13C NMR, GC-MS, etc).LUA is a neutral heterosaccharide. Its average molecular weight is 5166, it consists of arabinose, glucose and galactose. It contains (1â†'5)Ara, (1â†')Glu, (1â†'6)Gal and (1 â†'6)Glu, relative molecular ratio is 1: 2.4: 2.8: 7.6. IIID is an acidic heterosaccharide. Its molecular mass is 57143, it consists of rhamnose, glucose, galactose and glucuronic acid and contains (1â†'4)Gal, (1â†'6)Glu, (1,3â†'4)Rha and (1â†')Glu, relative molecular ratio is 1.9: 3.5: 1: 1.2. IIIF is a neutral heterosaccharide. Its molecular weight is 77103, it consists of arabinose, glucose, galactose and mannose. It contains(1â†'5)Ara, (1â†')Glu,(1—3)Man, (1—6)Glu and (1,4—6)Gal, relative molecular ratio is3.0: 2.4: h 7.2: 3.4. mj is a neutral heterosaccharide. Its molecular weight is 53010, it consists of arabinose, glucose, galactose, xylose, and mannose. It contains(l—5)Xyl, (1—5) Ara, (1—4)Man, (1—)Glu, (1—4)Gal and (1,3—6)Glu, relative molecular ratio is 1.4: 3.5: 1: 2.8: 3.7: 7.3. UIK is a neutral heterosaccharide. Its molecular weight is 4796, it consists of arabinose, glucose and galactose. It contains (1—) Ara, (1—4)Gal, (1—)Glu, (1,2—6) Glu and (1—6) Glu, relative molecular ratio ish 1.5: 1.8: 2.8: 2.5.