Autologous Bone Marrow Mesenchymal Stem Cells And Mononuclear Cell Transplantation In The Treatment Of Coronary Heart Disease Clinical Research

Objective: To establish the methods for culture of bone marrow mesenchymal stem cells (BMSCs) and mononucleus cells (BMMNCs). Method: Bone marrow mesenchymal stem cells were isolated from bone marrow by the methods of density centrifugation, cultured in medium (IMDM+20%FBS) for 3 weeks. Mononucleus cells were isolated from bone marrow by the methods of density centrifugation. Result: Bone marrow mesenchymal stem cells were spindle-shaped and adherea on the bottom of the flask after 48 ~ 72 hours' culture and increased after 2 ~ 3...