1. Study On Migration And Differentiation Of Bone Marrow Mesenchymal Stem Cells Transplantation Of Miniswines By Cardiac Catheterization In Coronary Artery 2. Study On Multidirectional Differentiation Of Bone Marrow Mesenchymal Stem Cells

Objective To establish a method of isolation and culture of mesenchymal stem cells(MSCs) from miniswines bone marrow and a model of miniswines by cardiac catheterization in coronary artery. Provided a new method of autologous MSCs transplantation to theat cardiovascular disease. And investigate the Migration and differentiation in cardiac muscle of autologous MSCs transplantation of miniswines by cardiac catheterization in coronary artery .Methods MSCs were isolated from bone marrow by extracting from the posterior superior iliac spine of miniswines and purified by centrifuge and cultured in single layer in vitro. MSCs quality were evaluated with morphology. By cardiac catheterization, cultured autologous MSCs in...