| Objective: To compare the distinction between Chinese traditional medicine and Western medicine on adjusting the function of heart immunity, antagonizing inflummation reactive scathe, reviving clinical symptom and toxin-side effect, et al. To explore unique superiority by the kind of Chinese traditional medicine of ABTBS that illustrate as guanxintongmai capcale(GXTM). To discuss medicine active mechanism on the facet in antagonizing heart inflammation immunoreation by using the kind of ABTBS. In order to expansive the study about affection of Chinese traditional medicine for ruling cardio-vascular disease field inflammation, illuminate the mechanism of antagonizing inflammation by using GXTM to prevention and cure coronary heart disease (CHD), and validate clinical curative effect and clinical applied security. It may establish base for applying GXTM to more extensive. It may sufficiently dig the developing potential of ABTBS in cardio-immunology field, and it also may afford the scientific theoretical evidence to developing new medicine mechanism of ABTBS.Method: we adopt two kinds of different research method, animal-experiment and clinic-obverse. (1)Animal -experiment study: Choose Wistar rat 100, make them experiment in order to filter the sensitive rat 50 to pit. We dispart stochastically them as vacant comparison group, model group, heat preconditioning group, western medicine group and TCM group, there are 10 rats every group. Heat preconditioning group before 24h complete heat preconditioning, afterward every group deal fractionated druggery or physiological brine, after 1h we anaesthetize the rats and introduce physiological two lead track record instrument to record the natural electrocardiogram, and then create the model by injecting pit in rats' belly, as the same time recording the electrocardiogram at different time, and take their blood by decollate rats head for inspect C-reactive protein, nitrogen oxide, malon double aldehyde, superoxide dismutase, creatine kinase; then cut rapidly the cardiac muscle of left ventricle tine to facture routine pathology slice for inspect extent of damnifid cardiac muscle by inflammation, and facture immune histochemistry slice for inspecting heat shock protein (HSP70), at one time exert multicolor phthology image meterage system to meterage HSP70 by half-ration meterage. By average ash-degree as half-ration parameter to mensurate the expression of HSP70. Data analysis adopt t-test, compare rate adopt X2-test. (2)Clinic research: The sufferer of CHD angina who examining at the first hospital of wuhanshi become research objects from 2004.2 to 2005.2, we select 60 from them as research object. Dispart averagely them intoGXTM group and west medicine group. Before give them experimented medicine we are stopping the medicine include MTC and the kind of nitric acid ester west medicine, and other routine medicinal treatment and work are not changing, and then GXTM group give them GXTM, west medicine group give them west medicinal commixture. A period of treatment is 4 weeks. As the same time we observe security of curative medication, clinical symptomatic, clinical curative effect, and examine IL-6, TNF-a, CRP before and after treatment. To analyze data by t-test, and to compare curative effect by Ridit analyse. Compare rate by X2-test.Result: (1)Experimented study result: Through comparing we can find HSP70 expression increasing distinctively in heat preconditioning group. There is also increasing trend in model group, it has different remarkably between vacant comparison group and model group, p<0.01. It may be related to acute ischemic and lack oxygen for myocardial. HSP70 of west medicine group also become increasing, but it has not different remarkably when it compares with model group. The reason maybe is homology with model group. HSP70 of GXTM group increases remarkably. It compares with heat preconditioning group that difference is remarkable, p<0.05. SOD's energy of heat preconditioning group, medicine group and model group has different each other, but they have no... |
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