| ObjectiveThe bcl-2 protein can restraint cell' s death. Transgenetic technology of bcl-2 gene can reduce infarct volumes. The research on transgenetic technology of bcl-2 gene therapy of the cerebral ischemic diseases is under investgation. There are two crucial problems in all transgenetic therapy: the pathway and the vector. In this study, we took pLXSN plasmid as vector and injected into the transient focal ischemic rat brain via ICA. We observed the protective effect of the bcl-2 gene in the rat model of transient focal ischemia. Hsp70 protein family , who are redundant in the stress cell, have been proved of the protective effect to the ischemic neurons. Some studies have found that the protective effect of the hsp70 protein family to the ischemic tissue in much extend were due to their function of antagonizing apoptosis. The relation between hsp70 and bcl-2 is beneficial to the further sdudy of the mechanism on the technology of bcl-2 gene transfering remedying cerebral ischemic . So this time we want to find if there is some relationship between the bcl-2 protein and hsp70 protein family, which will be the bases to the further research.MethodsConstructing the rat model of transient focal cerebral ischemia with the modified Longa method. We took out the suture after 2 hours. We injected pLX-SN plasmid (260ul) , pLXSN-bcl-2 plasmid (260ul) and normal saline into the rats'left internal carotid artery. All the survived rats were decapitated at 3 h,... |
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