Chantui Extract Active Sle Patients Lymphocyte Apoptosis Induced By Experimental Research

Objective: To study of apoptosis induced in lymphocyte from patients with active Systemic Lupus Erythematosus (SLE) by Cryptotympana Pustulata extract(CPE). Methods: By techniques of cell culture in vitro, lymphocyte from patients with active SLE were treated by CPE in 500ug/ml~4000ug/ml concentrations for 12h, 24h, 48h, 72h. Meanwhile two control groups were set up including normal group, CTX 200ug/ml. Then computed ratio of the cell survival by MTT. Observed and analyzed the ratio of the cell apoptosis, CD69 of expression by Flow-cytometry. Results: (l)500ug/ml~4000ug/ml CPE could induce lymphocyte from patients with active SLE apoptosis. The concentration of CPE showed a dose-and-time dependent effect.(2)The ratio of CD69 expression is 39.1%+1.6% when treated with 4000ug/ml CPE after 24h. Conclusion: It is found that CPE could induce lymphocyte from patients with active SLE activation to apoptosis.