| Objective To investigate the effect of Compound Peucedanum praeruptorum Dunn (CPd)on the pathophysiological changing and IL-6 and IL-8 level and the apoptosis correlated gene P53 protein expression of middle cerebral artery occlusion( MCAO) model. To study the mechanisms of CPd in preventing and treating ischemic apoplexy.Methods The model of focal cerebral ischemia/reperfusion in rats was established with the suture-occuluded method. To observe the effect of CPd on the behavior obstacle of MCAO rats, to study the bound of infarct and the pathological changing by the means of HE dyeing; to study the level of IL-6 and IL-8 in rats treated with CPd after the cerebral ischemia/ reperfusion were observed by immunaossay method; to study the level of gene P53 protein expression.Results After MCAO, different degree behavior obstacle appeared in all rats. The infarct spot can be observed in all groups except the sham group. The nerve cell necrosis can be finded in all rats after 72hr of MCAO/R. The result showed that 400% and 200% CPd can improve the behavior obstacle grade, reduce the extent of infarct bound obviously; After MCAO/R, the serum IL-6 and IL-8 were increased obviously but the CPd can reduced it;. After MCAO/R, the level of gene P53 protein expression were also increased obviously but the CPd can reduced it.Conclusion CPd can effectively reduced the extent of the bound of infarct, the excitable neural virulence and the depolarization aroud the infarct spot after cerebral ischemia, prevent and treat ischemic apoplexy better, may related to P53 antagonism and prevention of the cktokines such as IL-6 and IL-8 production after cerebral ischemia/reperfusion. |
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