Ea "gate", "absolutely Bone Of Ovariectomized Osteoporosis Preventive Effect Of The Experimental Study

Objective:Osteoporosis ( OP ) is syndrome shown on the bone absorptive rate is higher than formative rate during the metabolism of their bones. As the result, the progression of calcium deficiency and the degeneration of bone tissue are the characters in this period. Clinical manifestations appear on these elder persons, which are osteosclerosis and increasing the possibility of fracture. The approach used here is based on the former fundamental research. By using the molecular immunology technique, BMC ( Bond Mineral Content ) BMD ( Bond Mineral Density ) and IGF-I ( Insulin-like Growth Factor I ) was tested to study the mechanism of the electroacupuncture ( EA ) prophylaxis and treatment the OP. By conducting experiment, effects of different treatments were compared to see whether the difference exists, and provide experimental evident on clinical manifestation prophylaxis and treatment.Methods:In this experiment, 50 unfertilized female rats were chosen as the experimental objects, whose ages were all five-month-old and weight from 180 to 200 grams. By doing oophorectomy operations on 30 rats, fake operations on 10 rats, and non-operation on the rest of them as a normal group, the rats were randomly classified into three groups, 10 rats in each group. They were ovariectomized group, estrogen group, and EA group. Both EA group and estrogen groups were conducted treatments 90 days after the operations. All treatments were four-course. After the last treatment, the rats were anesthetized to test BMC BMD and taken blood by excising the rats' eyeballs. The blood was centrifugated to get about lml serum to test IGF-I (Insulin-like Growth Factor I ) by ELISA ( Enzyme-linked Immunoadsordent Assay) .Results:1. Both BMC and BMD of ovariectomized rats' lumbar vertebra and thighbone decreased ( P<0.01 ), so did IGF-I ( P<0.05 );2. Comparing with ovariectomized group, EA and estrogen could increase BMC and BMD of the lumbar vertebra and thighbone ( P<0.05 );3. Comparing with ovariectomized group, both EA and estrogen could enhance the level of IGF-I ( P<0.01 );Conclusion:According to the above results, both BMC and BMD of the ovariectomized rat's lumbar vertebra and thighbone decreased, at the same time, IGF-I decreased evidently. EA not only can help the ovariectomized rats to increase BMC and BMD of the lumbar vertebra and thighbone, but also to increase IGF-I contents to form bone cells and improve osteoporosis problem. Compared with estrogen, EA lowers the negative side-effect, provides the entire comprehensive functions of traditional Chinese medicine, and provides new theory on clinical treatment.