.1,25 (oh) <sub> 2 </ Sub> D <sub> 3 </ Sub> Research On The Role Of Immune Intervention In Nod Mice

objective:To investigate the effects of 1,25-dihydroxyvitamin D3 on diabetes in nonobese diabetic mice.The study aims at seeking the mechanism of 1,25-dihydroxyvitamin D3 preventing IDDM.Methods: Twenty femal NOD mice were divided into two equal groups. Group 1 received the intraperitoneal injection of peanut oil ;Group2 received the intraperitoneal injection of 1,25-dihydroxyvitamin D3 ever other day. Both groups mice were administered Cyclophosphamide(CYP) onset of the experiment.When experiment was finished we observed the incidence of diabetesand the degree of insulits.The expressions of bcl-2 and bax were detected with immunhistochemical technology . The rate of apoptosis of T lymphocyte was also measured by flow cytometry quantitative analysis.Results;Intraperitoneal injection of 1,25-dihydroxyvitamin D3 to NOD mice reduced the incidence of diabetes (10% vs 70% p<0.05). The insulits score and the severity of insulits in 1,25-dihydroxyvitamin D3 micewere lower than that of the control group(p<0.01). The rate of apoptosis of spleen lymphocyte was upper than that of the peanut oil group (32.31%±7.01% vs 24.19%±7.70%, p<0.05). The ratio of bcl-2/bax was upper than that of the control group(1.11 ±0.11 VS 0.87±0.16,P<0.01).Conclusion:These results show that 1,25-dihydroxyvitamin D3 could prevent NOD mice from developing into type 1 diabetes. It's mechanism may be related to regulating T cell immune.