| Lung cancer is one of the most common malignant tumor. For half a century the incidence of the primary lung cancer and the death rate have obviously risen in many countries and areas in the world. In our country lung cancer is the most common malignant tumor. As we know tumor cells have the ability to invade and metastate and the growth speed of the malignant cells is very high, so the therapy of the lung cancer is very difficult. Obviously, looking for effective method especially traditional Chinese medicine to treat lung cancer is very important for the management of cancer.Modern medical technologists consider recently that the happening of a cancer progression due to a lot of genes working together. In oncology research, focus which the researchers pay more attention for many years to find out that excessive proliferates and unusual splits of cells are the main etiology of tumor. With the appearance of the conception of apoptosis, we know that the insufficient apoptosis is one of the causes of tumor. Apoptosis is different from necrosis because it has a characteristic morphologic change. If apoptosis process is in an inordinate state, many disease including tumor may occur. Many researchers have indicated some antitumor drug can induce^ the malignant cells apoptosis.The vessel is the basic material for the growth of the tumor, and the growth of the tumor must rely on the vessel. With the increase of the vessel in the mesenchy of tumor, the..tumor grows more quicker. Not only the blood in the vessel bring enough nutrition and oxygen but also the vessel is the door that the malignant cells spread from the site of origin to distant sites. If no new vessels produce, the tumor tissue couldn' t grow after their diameter is 1mm or 2mm. So if there are more vessels , the malignant tumor will grow more quickly and invade the tissues nearby and then metastate. The microvessle density is used to indicate, the tumor characteristics.It is effective to treat lung cancer with yiqihuoxuejiedu herb, but there are no reports in the morphologic changes by microscope-electron microscope and in the changes of enzyme activity and induced apoptosis. Microscope-enzyme cell chemical staining and transmission electron microscope technique were used in ourexperiment, which compared yiqihuoxuejiedu herb to shun-bo, observed reaction of mice lung cancer of Lewis cells. In this experiment, we use the mouse (C57BL\6 ) to form the animal model of lung cancer . After 24 hours, we begin to give drug for 15 days: the compared group give 0.5ml N. S. 0.9% every, mouse by pour, the traditional Chinese medicine group give 0, 5ml; shun-bo 1mg/kg every mouse. The grain of Mg2+-ATPase which is the sign enzyme of plasmalemma and the grain of G-6-Pase which is sign enzyme of endoplasmic reticulum changed small, the quantity and density of the grain reduced, enzyme activation was came down. With light microscope, we can observe the microvessel density is decrease. Morphological examination by electron microscope showed the presence of "apoptotic bodies" . .Conclision: (1)Yiqi huoxue jiedu herb can restrain the growth of the lung cancer. (2)Yiqi huoxue jiedu herb can induce apoptosis of the lung cancer cell. (3)Yiqi huoxue jiedu herb can reduce Mg2+-ATPase and G-6-Pase enzyme activation. @Yiqi huoxue jiedu herb can decrease the microvessel density . |
PDF Full Text Request