| IntroductionParkman found that CD43 presenting abnormally is closely related with Wiskott - Ablrich which is X -linked recessive disease in 1981. And in 1990 Ardman et al detected autoantibody of anti - CD43 in patients of infecting HIV - 1 and aroused more interest about CD43. It is reported that The expression of CD43 on the peripheral blood T lymphocyte in HIV patients is abnormal and CD43 electrophoretic migration rate alter in 2000. It is also found that CD43 play an important regulating fuction in HIV special reaction.CD43 antigen has been cloned, but it has no autoploidy with proteins which have been known. CD43 locates in chromosome 16P11.20 Recently, Studies shows that chromosome 16pl2 - 11 can enhance IgE allergic reaction , while the gene region coding CD43 locates in it. As a result it is surposed that CD43 is important in regulating human plas-mocyte,T lymphocyte and B lymphocyte. Since CD43 is found abnomal in various immunological deficiency diseases,it is thought as an allergic reaction candidate gene.CD43 (leukosialin) is also called sialophrin. It is an integrate adhesion protein on the surface of cell and is presented on the surface of yolk sac cell, fetus liver cell and bone marrow cell of embryonic phase. Inadult,CD43 is presented on hemopoietic stem cell and all the white cell except for native B lymphocyte. CD43 is also presente on the surface of histamine macrophage , dendritic cell, epithelial cell and endothelial cell. It is also found CD43 is presented on the surface leucoma cell, solid tumour and some types of leukemia cell. SinceCD43 is distribute so widely, it is surposed that it play an important role in interaction among cells and in immunological regulation. But studies about CD43 have not been developed completely so far. And there is neccessity to investigate iy deeply.Materials and MethodsUsing indirect immunofluorescent marker - flow cytometry, we examined the percentage of CD43 + CD19+ Cell. The IgG and IgM of the serum was tested with ELISA.Results1. This study demonstrates that CD43 on B lymphocyte increasely after being stimulated by PWM using double labelled flow cytometry method. The percentage of CD43 * CD19 + are significantly higer than other groups when tested on 48 hours. There are no significance between experimental group and cotrols. When tested on 24 and 96 hours. The B cells aptosis when tested on 144 and 168 hours. It can' t meet the quantity of cells which FACS needs. So we can' t test the two control group. There are no significance between experimental group when tested on 24 hour, 96 hour 144hour and 168 hour. There are no significance between controls.2. The IgG and IgM production are significantly higher than controls. There are no correlation between Ig serection and the CD43 expression of B cells.3. Anti - CD43 mAb can lead to the aggreation of the lymphocytes.4. Anti - CD43 mAb can't alter the Ig secretion in PWM - induced system.DisscussionIt is reported that differrent phrase B lymphocyte can be divided into CD43 + B and CD43 ~ B cell cluster with anti - CD43 monoclone antibody. So it is surposed that CD43 + B cell represent an independent B cell lineage just as CD5+ B cell. This experiment shows that B cell expressing CD43 increses in the next day after being stimmulated by PWM. This result confirmed that CD43 is a surface marker of B cell expressed increasely after the activation and differentiation of B cell. So the notion that CD43 + B cell represent an independent B cell lineage is not reasonable.To messure the frenqency of CD43 on the surface of B lymphocyte correctly in this experiment, every cell sample is divided into two part and each part has a self - control, so we can decrease the non ?specific strain to the least.M. Wiken et al. reported that the percentage of CD43 + B cell in the peripherical B cell is 12% - 80% after being stimulated by TPA or anti - CD43 nonoclone antibody using indirect - labelled. (when self T cell and 5 percent monocyte are present ) . If this percen... |
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