| ObjectiveIncidence of Benign prostate hyperplasia ( BPH) in man over 50 years old is 50 -80% , and that of Prostate cancer (PC) increased considerably in China recently. However , the mechanisms of BPH and PC are not well understood. So it's necessary to investigate the molecular - biological mechanisms leading to preceding prostate diseases for their treatments.Mitogen - activated protein kinases ( MAPKs ) can be classified into three classes; extracellular signal -regulated kinase( ERK1/2) , stress -activated protein kinase(SAPK)/ c -Jun N -terminal kinase ( JNK) and P38MAPK. MAPKs play a very important role in inducing the process of cell growth, development, division, differentiation and apoptosis. Abnormity of MAPK signal - transmitting pathway certainly will cause imbalance between cell proliferation and apoptosis, thus lead to hyperplasia of cell which is accord with the process of BPH and PC.To discuss the functions of ERK, JNK and P38 in the process of formation of BPH and PC by examining the expressions of them in tissues of benign prostate hyperplasia, prostate cancer and normal prostate, so to provide basis for the molecular mechanism of BPH and PC.MethodImmunohistochemistry method is utilized to examine the expres-sion of ERK, JNK and P38 MAPK protein in nucleus in tissues of benign prostate hyperplasia, prostate cancer and normal prostate. Results determining: it's commonly admitted that MAPK mainly located in nulei to express their biological effects, so the number of brown -dyed nuclei is made as counting standard for positive cell. 200 epithelial and stromal cells were counted in 5 randomly observed visual fields each slide. Ratio of positive cell is calculated. Statistical methods : data is expressed in the form of ( x + s) , each group were compared by ANOVA, and the significance of differences between groups was elavuated by The Fisher and Behrens test.ResultsERK is expressed in both plasma and nuleus of stromal and epithelial cells in BPH and PC with different degrees. ERK is expressed in both plasma and nuleus only of stromal cells, while in epithelial cells, its only in plasma.Positive ratio of ERK in stromal cells nucleus is 79% in BPH, 50% in PC, 48% in normal prostate tissue. There exists significant difference between BPH and the latter two (P <0.05) , while there is no difference between PC and normal prostate tissue(P >0.05). Positive ratio of ERK in epidermal cell nucleus is 72% in BPH, 90% in PC, and 0 in normal prostate tissue. There is significant difference a-mong those three kinds of tissue( P <0. 05).JNK is expressed in both plasma and nucleus of metrical and epidermal cell in BPH , PC and normal tissue, but the expression in nucleus is low. There exists no significant difference among them .Expressions of P38 in epithelial cells in normal prostate and BPHtissue are 28% and 32% respectively, while 25% and 21% in stro-mal cells. There is no difference between them. Expressions of P38 in epidermal cells in PC increased considerably, and it's about 57% , while in stromal cells, its only about 15% . There is significant difference between PC and normal tissue(P < 0. 05).Conclusionfollowing conclusions are drawn according to our study:(1) ERK may be a main signal - transmitting pathway in the occurrence and development of BPH and PC.(2)JNK/SAPK may be not involved in the pathological mechanism of the occurrence of BPH and PC.(3) relatively decreased expression of P38 may play a role in BPH.( 4 ) regulating the expressions of ERK and P38 may be helpful for the treatment of BPH and PC. |
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