From Hippocampal Slices Extracellular Recording And Hplc In The Study Of Anoxic Brain Damage

Ischemic stroke is a leading cause of death and serious, long-term disability in adults. Now researchers are studying the mechanisms of the disease and ways to prevent this injury to the brain.Using rat hippocampal slices ischemia model in vitro, We set up the method of extracellular recording technique and amino acids detection with the HPLC system to study brain hypoxic injury. With the method , We studied the neuroprotective effects of Calmodulin(CaM) antagonist trifluoperation(TFP) against hypoxic injury.The changes of PS after hypoxia and the protective effects of TFP were studied by the extracellular recording technique in rat hippocampal slices. There is no report to show that this sort of experiment has ever been done elsewhere. The results showed that treated with TFP, the disappearance time of PS after hypoxia was significantly suspended, the appearance of hypoxic injury potential (HIP) was lower and the recovery amplitude of PS after reoxygenation was significantly higher than that of OGD group. The results showed that TFP can protect brain slices from hypoxic injury.With the method of amino acids detection in the culture medium of brain slices with the HPLC system. We studied the effect of TFP on the changes of level of amino acids (Asp, Glu, Tau, GABA) in the culture medium of brain slices during OGD. It was found that OGD increase the release of amino acids and TFP can markedly inhibit the increase of the excitatory amino acids (EAAs).The extracellular recording technique in rat hippocampal slices , associated with the HPLC fluoromatric method would be helpful for further studies in pathological mechanisms to ischemic stroke.The study also showed that TFP can protect brain from hypoxic injury . The mechanism may be that TFP can reduce the increase of excitatory amino acids and protect synapyic function from hypoxic injury.