Leukemia Patients On The 11th Chromosome Micro-satellite Instability And Loss Of Heterozygosity Of The Application

Abstract Objective To detect the frequency of microsatellite instability(MSI) and loss of heterozygosity (LOH), to investigate the function of LOH and br MSI during the progress of pathogenesis in leukemia. Methods PCR. amplification of 4 microsatellite loci at chromsome lip in 30 leukemias, (including 18 acute leukemia, 12 chronic leukemia). To analyze the production for LOH and br MSI by electrophoresis on polyacrylamide gels, argentine dye. Results 9 of 18 acute leukemias demonstrated LOH at one or more loci . The frequency of LOH on D11S922~. D11S13311 D11S1334 D11S929 is 22.2% (4/18)~ 22.2% (4/18)~ 27.8% (5/18) and 16.7% (3/18)respectively. None of informative patients in chronic leukemia showed LOH. 2 of 30 patients showed MSI on the selected loci and one is AL, the other is CL. The later with MSI on two loci occurred acute myeloid leukemia after three months. Conclusion The frequency of LOH on D11S9221 D11S1331 D11S1334 and D11S929 in acute leukemia is markedly higher than that of in chronic leukemia that suggests the presence of one or more tumor suppressor gene in the 1 lpl4-pl5 region which associated with the progression of AL. To detect more microsatellite loci in this region may identif~r and locate the candidate tumor suppressor gene. Our results indicate that MSI on loci of iipi 4-15 be uncommon events in leukemia.