The Study Of Microsatellite Alterations On Chromsome 8 In Colorectal Cancer

Objective To study the frequency of MA on 8p , to investigate the function of MA during the progress of pathogenesis in colorectal cancer. Methods By PCR, polyacrylamide gel-electrophoresis and silver staining, MA was detected at 4 microsatellite loci on chromsome 8p in tumor tissues,adjacent tissues and normal colorectum tissues excised from 34 cases with colorectal cancer . Results (T) 14 cases demonstrated MA at one or more loci ,the total frequency was 41.18%. The frequency of MA on D8S135. D8S255, D8S51K D8S137was 29.41%x 20.59%> 17.65%^ 11.76%., respectively. @ MA positive at 2 and more loci was 10, the frequency among the total positive cases was71.42% . None patient's adjacent tissues was found MA, Conclusions (1) The most common MA occurrence at the 4 loci might imply the existence of the potential genes related to the carcinogenesis of colorectal cancer, and provide a effective index for early diagnosis ofcolonrectal cancer. (2)The MA was not found in the 34 selected patient's adjacent tissues.