P-selectin In Tongue Squamous Cell Carcinoma Metastasis In The Relationship Between Research,

AbstractDepartment of Oral Clinical Medicine, Medical College, Qingdao University Postgraduate Cai ZhenInstructor Fan Gongwei, Chen WantaoObjective To study the molecular basis of tumor cell translocation from the aspects of P-selectin mediating of tumor adhesion,in order to make known the secrets of malignant tumor cell metastasis, which can provide new ways block the metastasis of malignant tumor . Methods Using lipoprotein mediated DNA translocation the CHO cells were transfected with P-selectin, then selected by G418 and MTX and detected by Flow-type cell instrument, in order to obtain the highly -expressed P-CHO cells. By the Rolling Test ,we detected the rolling and binding of tongue squamous cell carcinoma cells with CHO cells which have P-selectin expression. Results Using the me- thod, we can obtain the highly expressed P-CHO cells. In the Rolling Test, the number of Tca8113 cells adhering to P-CHO com- pare with the number of Tca8113 cells adhering to CHO has statistical signi- ficance (P<0.001). After P-selectin adhesion-blockage monoclonal antibody and polyclonal antibody handling, the adherance of Tca 8113 cells with CHO has no changes (p>0.05). the number of Tca8113 ,s adherance to P-CHO after mAbG1 handling have statisti- cal significance (P<0.001), the number of adherance after PolyAb handling also have statistical significance (P<0.001), and the num- ber of Tca8113 cells adhering to P-CHO and CHO after mAbPS1 has no changes than before(p>0.05). Conclusion 1 Using cell culture study infiltrative growth and metastasis is better than using assay in vivo. 2 Flow cytometric assay is very important method to detect P-selectin Rg adhere CHO cells. 3Using lipoprotein mediated DNA translocation the CHO cells transfected with P-selectin, then select- ed by MTX and detected by Flow-type cell instrument, is a better way to obtain the highly -expressed P-CHO cells. 4.The adhering of Tca8113 cells to P-selecting has specificity and it has significant dep- endency on P-selectin. It can be concluded that P-selectin plan an important role in metastasis of human oral malignant tumor and it play a significant role in finding a good way to block the adhesion and metastasis of tumor.