| The traditional herb, Phyllanthus amarus(P.amaurs), distributes most parts of China, which has the effects of quenching evil heat in the liver, activating the blood and toxin-removing and has a long history of treatment many diseases, such as icterus. The compound of P.amaurs has been studied in lab, observed at hospital for many years and selected again and again and at last formed after many times improvement by Tropical Medicine Institute of Guangzhou University of TCM. Which is made up of P.amaurs, Astragalus membranaceus and Panax notoginseng, etc and is useful in the treatment of chronic hepatitis B virus infection and has the effects of clearing away heat and toxic materials, soothe the liver and invigorate the spleen and activating the blood circulation to eliminate blood stasis. DHBV model is an effective way to appraise the anti-HBV drug screening test at present. For being lack of stability and easily affected by toxin, the acquired infection DHBV ducks is not a good way to appraise the drug effect, so it is necessary to build a more stable and effective DHBV model. Spot-blot hybridization is widely used in this experiment. Some experiment conditions will be changed so that we can get the real data about the change of DHBV-DNA in peripheral blood of Guangzhou Brown spot ducks. Directly detect DHBV-DNA in peripheral blood of Guangzhou Brown spot ducks by spot-blot Hybridization method, view it as positive when spot appears, take 0D490 outcome as the level of DHB V-DNA, collect blood once every ten days, observe 90 days continually. The results showed that the natural infection rate of Guangzhou Brown spot Ducks was 37.66%, the level of DHBV-DNA was higher and lasted lOnger, so it is good as a congenital infection DHBV animal and on this basis we build congenital infection DHBV model. Through initial observation the season, food and DHBV positive serum do almost affect the level of DHBV-DNA in peripheral blood of Guangzhou Brown spot Ducks. This study suggested that this mode might be a good DHBV model for its economy, simplicity and good stability. This study observed the anti-DHBV effect of P.amaurs and its compound in Guangzhou Brown spot Ducks with congenital and acquired infection of DHBV. The results showed Hainan P.amaurs, Fujian P.amaurs and Guangxi P.amaurs are all useful, at the same time, also found the same drug, the same dosage, have the different effect, the inhibition tODHBV-DNA in acquired ducks is stronger than in congenital ducks. This maybe relative to high level DHBV-DNA, lasting longer time and the endurance of immunity. 3TC and Fujian P.amaurs used alone have a strong inhibition to DHBV-DNA, both used together did not show the additional effect of anti-DHBV, it appears contradict to clinic; 3TC's inhibition to DHBV easily fluctuates, but ACV's effect is stable. Now we are not quite sure whether it is relative to the bad stability and easy dissolution or not. We use the compound of P.amaurs and Anti Hepatitis B specific transfer factor together treating 40 chronic hepatitis B patients, and take the compound of P.amaurs alone as control class. The results showed the whole efficiency of the therapy group is 92.5%, HBeAg seroconversion is 55.5%, HBV-DNA seroconversion is 56.2%; the control class is 73.3%, HBeAg seroconversion is 44.4%, HBV-DNA seroconversion is 43.4%. |
