The. Cyclovirobuxine-d Protective Effect On Cerebral Ischemic Injury In

CBV-D has been recorded by Chinese Phamacopoeia. The effects of CBV-D on cerebral ischemia reperfusion were studied in this artiele.This dissertation consists of three patrs. Part 1. Effect of CBV-D on micro-circulation: The effect of CVB-D was studied from the following aspects: experiment of micro-circulation on the auricle of mice, rabbit conjunctiva aperture of and obstruction of meninges micro-circulation of rats induced by urgent in-complete ischemia. The results showed that CVB-D could dilate micro-artery and micro-vena, improve line and particle flow. Therefore, the CBV-D can markedly improved micro-circulation. CBV-D dilate micro-artery and micro-vena on auricle of mice administrated 10mg. kg-1, 20. 0mg. kg-1 by 1. g, once a day for three day. However there isn?t marked influence on flow of blood. But it could markedly increase both flow velocity of blood and flow velocity of blood induced by Dextran T500 conjunctiva of aperture on rabbit; l0mg.kg?20.Omg.kg CBV-D can markedly dilate micro-artery. The decreased flow velocity of blood induced by urgent non-complete ischemia on rats can increase, simultaneously improve contractions on meninges micro-artery. Simultaneity , the results shows that effects of CBV-D on micro-circulation are diffenent to varsity. Part 2 Effect of CBV-D against cerebral ischemia-reperfusion injury in mice: On the cerebral ischemia-reperfusion model mice, the levels of MDA,NO and the activity of NOS were increased, but the activity of LDH were decreased, and the water content of brain ,index of brain and the wet weight of brain were increased. 5 mg. kg-1 10mg. kg1CBV-D could improve the learning and memory capability of model mice in step down test, but there were not markedly improve at the dosage of 20mg. kg CBV-D.5mg. kg-1,10mg. kg-1CBV-D could markedly decreased index of brain, water content of brain and wet weight of brain, 2Omg.kg-1CBV-D could not markedly. 10mg.kg-1CBV-D could markedly improved SOD, LDH, MBA and NO. 20mg. k&tCBV-D could markedly improve SOB, however 5mg.kg-1CBV-D could markedly improve LDH. Part3. Protective effects on H2O2-induced ,Glu-induced and ischemic injury in culturing rat cortical neurons: The efflux of LDH of neurons, content of MBA, NO in neurons and activity of NOS were increased by H202-induced ,Glu-induced and ischemic injury in culturing rat cortical neurons. 2OX1O-1 CBV-D could markedly reduced the effux of LDH and content of MBA, which results showed that CBV-D had perfect effect on 1-1202-induced cultured rat cortical neurons. To Glu-induced in cultured rat cortical neurons, 1OXlO-1 CBV-D and 2Oy10-1 CBV-D could markedly reduce the effux of LDH and content of MBA, 20/lW CBV-D could markedly reduce activity of NOS and content of NO. To ischemic injury in cultured rat cortical neurons, three groups of CBV-D could markedly reduce the effux of LDH; 20/lW CBV-D could markedly reduce activity of NOS, content of NO and content of MBA. iO-1iW CBV-D could markedly reduced content of NO and content of MDA. 20/lW CBV-D could markedly reduced activity of NOS. Both iOyiW CBV-D and20/1W CBV-D can reduce the cell death ratio.