Resistance To Deformation Of Animal Studies In Streptococcus Chicken Egg Yolk Antibodies To Prevent Dental Caries

Objective: The primary extract of anti-streptococcus nzulans IgY was prepared and observed it-s pretective effect on rats-dental caries The anti-ultreptococcus znulans IgY was primary extracted from the eggs laid by the hens immunized by &rnutans and S. sobrinus with adjuvant by water dilution ultrafiltration method.The protein content of the anti-ultreptococcus inut~zns IgY was determined by Lowray's method. The titer of the anti-ultreptococcus rniitans IgY was examined by Passive JIemagglutination Assay(PIIA). 40 S-D rats have been established dental caries model were divided into four groups with random block degisn: the first group was given 2% the anti-ultreptococcus nzutans IgY in daily drinks; the second group was given lpptn sodium fluoride; the third group was given the non-special IgY extracted by the same method from the eggs laid by the hens without iniinunity ; the fourth group was given 4 distilled water. After 55 days of treatment, the caries scores were measured by the keyes's method. Results: The anti-ultreptococcus fihlltans IgY was produced in eggs laid by hens after immunity. The protein content of primary extract of anti-ultreptococcus nzutans IgY extracted from eggs yolk by water dilution ultrafiltration method was 100mg/ml. The titer of this TgY was 1:512. The keyes' caries score of the first group was significant lower than the one of the third group and the fourth group(p<0.01) . The scores of the second group was significant lower than it of the third group and the fourth group (p<0.01) too. There wasn't significant difference between the scores of the first group and the second group. Conclusion:The primary extract of anti-ultrpotococcus znutans JgY extracted by the method of this study could effective prctected rats against dental caries.The effect was simi larly with sodium fiuorid.