Neurovascular Reconstruction Promoting Function Of Ω-3 Polyunsaturated Fatty Acids In Neonatal Rat Brain After Hypoxic Ischemic Damage

Neonatal hypoxic ischemic encephalopathy (hypoxic-ischemic encephalopathy, HIE) is the most common perinatal cause brain damage, not only threaten the lives of newborns, but also a serious neurological sequelae. However, in neonates with hypoxic ischemic encephalopathy in the treatment, so far there is no unity at home and abroad, effective treatment. Therefore, the search for treatment of neonatal hypoxic ischemic encephalopathy has become very important in the medical workers. Neonatal rat cerebral hypoxia-ischemia models are widely used to study neonatal brain injury in the pathogenesis of hypoxic ischemic, early diagnosis and intervention methods of screening like evaluation.Fish oil rich inΩ-3 polyunsaturated fatty acids (mainly docosahexaenoic acid, DHA. and eicosapentaenoic acid, EPA).Ω-3 PUFAs have long been known to be important for nervous tissue growth and function in the developing brain. MaternalΩ-3 PUFAs deficiency alter neurogenesis in the embryonic rat brain in a pattern suggesting impaired post-mitotic cell migration. Recent studies further suggest that the adult brain ofΩ-3 PUFA and nerve regeneration are likely to be relevant. Adding DHA in food can promote the young and aged rats hippocampus neurogenesis and improve learning and memory. Previous studies found that DHA in adult rats with ischemic brain injury in the significant neuroprotective effects, but the mechanism is unclear. In recent years, study found that angiogenesis and nerve regeneration (neurogenesis) are two important mechanisms in the brain repair after stroke. How to enhance angiogenesis and nerve regeneration to improve the recovery of neurological function after stroke has become a research hot. Therefor, in this study we used the neonatal hpoxic/ischemic (H/I) model by cresyl violet staining assessment of ischemic infarct volume; immunofluorescence staining, confocal imange methods assessment of nerve regeneration and angiogenesis, and the sensory-motor learning and memory behavior tests, we observed the fish oil has the long-term protective effect via promoting nerve regeneration and angiogenesis in neonatal hypoxic ischemic injury.Result:1. Dietary supplyment fish oil before and after H/I insult decrease the cerebral tissue loss and improves neurological outcomes 7 days after.2. In the long-term recovery experimental paradigms (5 weeks after H/I), the neuroprotective effect ofΩ-3PUFAs remained.5 weeks later,Ω-3PUFAs also ameliorated cerebral infact and behavioral deficits.3.Ω2-3PUFAs enhance cell proliferation,and neuron replacement after hypoxia/ischemia.4.Ω2-3PUFAs enhance functional revascularization after hypoxia/ischemia.Conclusion:Ω-3PUFAs may provide the neuroprotective effect against hypoxia-ischemia via neurogenesis and angiogenesis.