| Acupuncture reinforcement and reduction is not only the important part of acupuncture manipulation, but also the key of obtaining curative effect in clinical. Acupuncture lifting-thrusting reinforcement and reduction is a single manipulation of reinforcement and reduction, which is the most basic and the most commonly used, so it is always valued by doctors historically because of its theoretical value, and the clinical guide significance. But doctors of later generations have divergences about lifting-thrusting reinforcement and reduction due to the different emphasis of the discussions on it through the ages. Therefore, using modern experimental research method to demonstrate effectively lifting-thrusting reinforcement and reduction is necessary completely.Objective:(1) To study whether the effect is different between lifting-thrusting reinforcement method and lifting-thrusting reduction method on blood-deficiency rabbits; (2) To study whether the effect of lifting-thrusting reinforcement method is better than lifting-thrusting reduction method on blood-deficiency rabbits; (3) To evaluate objectively acupuncture manipulation of lifting-thrusting reinforcement and reduction.Methods:Sixty rabbits were randomly and evenly assigned to five groups:A group (blank group), B group (model control group), C group (retaining needle group), D group (lifting-thrusting reinforcement group), E group (lifting-thrusting reduction group). The last three groups were called treating groups. The A group was not done any treatment, just synchronously fed. Besides A group, the rabbits of other four groups were established blood-deficiency model by bleeding and restricting diet. After making blood-deficiency model, B group:just bound without any treatment and recover spontaneously; C group:acupuncture both "Zusanli" (ST 36) of rabbits without any reinforcing and reducing methods and retain needle for 15 minutes; D group: acupuncture both "Zusanli" (ST 36) of rabbits with lifting-thrusting reinforcement method in accordance with prerecorded manipulating word of command; E group: acupuncture both "Zusanli" (ST 36) of rabbits with lifting-thrusting reduction method in accordance with prerecorded manipulating word of command. Do treatment every other day,10 times totally. Blood samples were collected from the ear margin veins for examine RBC and Hb at both before and after making blood-deficiency model and also after acupuncture treatment of the 2 day,the 17 day and the 32 day respectively. Use the SAS 8.2 statistical software for statistical analysis of data.Results:(1) Before making blood-deficiency model, there are no difference both RBC and Hb among five groups, P>0.05. It indicated that all five groups were not significant different and could be compared with each other.After making blood-deficiency model, values of RBC and Hb of B group, C group, D group and E group which were made blood-deficiency model all decreased obviously. Compared with before making blood-deficiency model respectively, the difference of the four groups were all statistically significant P<0.05 and the decrease of both RBC and Hb were more than 20%. In addition, after making blood-deficiency model there were significant different between four groups which were made blood-deficiency model and blank group, P<0.05. It indicated that we succeeded in establishing blood-deficiency model by bleeding and restricting diet.Before acupuncture treatment, there are no difference both RBC and Hb among B group, C group, D group and E group, P>0.05. It indicated that all four groups were not significant different and could be compared with each other.(2) After acupuncture treatment, blood samples were collected from the ear margin veins for examine RBC and Hb at the 2 day,the 17 day and the 32 day respectively. It showed that values of RBC and Hb of B group, C group, D group and E group all increased obviously. Compared with RBC and Hb before acupuncture treatment respectively, the difference of the four groups were all statistically significant,P<0.05. Compared with RBC and Hb among after acupuncture treatment of the 2 day, the 17 day and the 32 day of B group, C group, D group and E group respectively, it showed that there were not significant difference among the three time points in each one of the four groups, P>0.05. Compared with RBC and Hb among B group, C group, D group and E group after acupuncture treatment of the 2 day,the 17 day and the 32 day respectively, it showed that there were not significant difference among the four groups at each one of the three time points.Conclusion:(1) In this experiment, there was no significant difference between lifting-thrusting reinforcement group with thrusting heavy and lifting light and lifting-thrusting reduction group with lifting heavy and thrusting light in the weak condition, and it didn't showed effect of reinforcement or reduction. It indicated that it might be related to the same quantity of stimulus produced by operation. (2) In this experiment, we observed model control group and treating groups. The results showed that there was no significant different effect between treating groups with acupuncture and model control group with spontaneous recovery. It indicated that maybe body condition of deficiency or excess is the main influencing factor of producing reinforcement effect or reduction effect in acupuncture therapeutic process. (3) Lifting-thrusting reinforcement and reduction method was quantized precisely from the frequency and the amplitude in experiment.,which could be seen as preliminary discussion to the standard of acupuncture reinforcement and reduction method. |
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