| Being a traditional Chinese medicine treatment, acupuncture has a remarkable effect on psoriasis. Our study is to observe the pathological change of electroacupuncture on psoriasis model, exploring the therapeutic mechanism of acupuncture for psoriasis, and to supply evidence for the expanding application of electroacupuncture therapy in treating psoriasis. This thesis contains literature review and experimental research.Literature Review is divided into the clinical research progress of acupuncture treatment and the Animal Models of psoriasis. With regards to the overview of clinical study on acupuncture treatment of psoriasis, this paper summaries the selection methods of acupuncture point and treatment equipment. Besides, this article illustrates the common types of Animal models, experimental methods, pharmacological & pharmacokinetic and efficacy characteristics.Purpose:The aims of the paper are to study the influence of electro-acupuncture (EA) on the guinea pig model with psoriasis. We examined the treatment of psoriasis by using EA, evaluated its efficacy and explored the mechanism. Additionally, we compared the differences between symptoms before and after acupuncture treatment, and between groups to better understand its mechanisms. Methods:30 guinea pigs were randomly and evenly divided into 3 groups:the control group, the observation group and the EA treatment group. The observation group and the EA treatment group were given 5% Propranolol liniment on ear skin to generate psoriasis-like changes as guinea pig models of psoriasis, while no intervention was conducted in control group. EA treatment group received EA treatment, while the control group and the observation group didn't receive any treatment. Then local skin lesions and pathological changes were observed and were compared among groups.Results:Before treatment, the control group showed smooth and meager, pale pink, hair thick and shiny, close to the skin, while the two groups which were given 5% propranolol liniment appeared significant and psoriasis-like changes on ears. After treatment, the observation group performed as parts of losing hair or no hair roots, as well as some parts of the ear skin were red, were observed visible dark erythema, hypertrophy, overlying silver-white scales, and telangiectasia compared to the control group; EA treatment group showed bilateral auricular skin returned to normal, while those in control group did not improve. The ear skin changes in control group can be seen by using microscopy, such as meager stratum corneum, about 1 to 3 granular layers,3-5 layers of polygonal acanthae cells, simple column basal cells, epidermal suddens flat, sparse scattered superficial dermal infiltration of lymphocytes, and unobvious telangiectasia, etc; in observation group, hyperkeratosis and parakeratosis as well as Munro micro-abscesses were found, granular layer became thinner or disappeared, hypertrophic acanthosis fluctuated, and capilliaries in superficial layer and papilla expanded and congested; in EA treatment group, slight parakeratosis was observed, spinous laye became thinner, the epidermis flatted or almost disappeared, a small amount of dermal capilliarie expanded, mononuclear and polymorphonuclear cell infiltrated less around vessels, and Muni micro-abscesses have not been found yet. The pathological appearances of skin lesions on guinea pig were in accordance with the histopathology performance of psoriasis in observation group and the E treatment group. Furthermore, histological Baker score and inflammatory cell infiltration scol increased significantly compared to those in the control group (P<0.01), which demonstrated tr guinea pig model can be used in studying psoriasis. On the contrary, Baker score and inflammatoi cell infiltration score of the EA treatment group was significantly lower than the observation group(( <0.01). Additionally, histo-pathological changes were not found in major organs of guinea pigs.Conclusion:The guinea pig model can be used to generate psoriasis-like skin changes, and also EA recognized as a safe and effective treatment to improve ear skin lesions and pathological changes c the guinea pig with psoriasis. |
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