Soymilk And Fermented Soymilk On The Preventive Effect Of Nonalcoholic Fatty Liver

ObjectiObjective:To study the influence of soy milk and fermented soy milk on the function ofnonalcoholic fatty liver disease (NAFLD) rats. Blood biochemical parameters andliver peroxisome proliferators activated receptorsα(PPARα) mRNA and its targetgene carnitine palmityltransferase-1 (CPT-1) in rats were detected, then explore thetherapeutic effect of soy milk and fermented soy milk on NAFLD rats.Methods:48 male Sprague-Dawley (SD) rats was divided into A (basic feed group), B (high- fatdiet group), C (high-fat diet +high dose soy milk group), D (high-fat diet+low dosesoy milk group), E (high-fat diet+high dose fermented soy milk group), F (high-fatdiet+low dose fermented soybean milk group) by weight and serum total cholesterollevel. Indexes as follow were checked at the end of this experience: Serum totalcholesterol (TC), triglyceride (TG), high density lipoprotein- cholesterol (HDL-C),low density lipoprotein-cholesterol (LDL-C).8 weeks later, all rats were sacrificed todetect levels of liver PPARαand CPT-1 by reverse transcription-polymerase chainreaction (RT-PCR).Results:1.After feeding 21 days, the serum cholesterol level of other groups increasedcompared with the basic feed group (P<0.01).2.The liver index level of soy milk groups and fermented soy milk groups were higherthan that of high-fat diet group (P<0.01), but lower than that of basic feed group(P<0.05).3.At the end of this experiment, the serum total cholesterol level of all experimentalgroups were significantly lower than the model group (P<0.05). The high-densitylipoprotein levels of all experimental groups had no significant difference (P＞0.05).The serum Triglyceride level of high-fat diet+low dose soy milk group was higherthan that of high-fat diet group (P<0.05). The serum Triglyceride levels of high-fatdiet+low dose soy milk group and high-fat diet+high dose fermented soy milk groupwere higher than that of high-fat diet group (P<0.05).4．According to Liver biopsy, we could see that all experimental groups had steatosis,but not as seriously as high-fat diet group (P<0.05). Furthermore, on the basis of liversteatosis points, all experimental groups had lower score than high-fat diet group(P<0.05). 5.Compared with high-fat diet group,free fatty acid decreased in all experimentalgroups (P<0.01).6.Compared with high-fat diet group, PPARαmRNA expression increasedsignificantly in the high-fat diet+high dose soy milk group and the high-fat diet+highdose fermented soy milk group (P<0.05). Compared with basic feed group, PPARαmRNA expression increased remarkably in all experimental groups (P<0.05). TheCPT-1 mRNA expression levels of all experimental groups were significantly higherthan that of high-fat diet group (P<0.05).Conclusion:1.Rat model of NAFLD was induced successfully by feeding with high-fat diet.2.Soy milk and fermented soy milk reduced serum total cholesterol level, but did notrise the serum HDL-C level.3.Soy milk and fermented soy milk remarkably decreased the level of NAFLD ratliver index.4.High dose soy milk and high dose fermented soy milk could up-regulate the PPARαmRNA expression levels.5.Soy milk and fermented soy milk could up-regulate the CPT-1 mRNA expressionlevels.6.Soy milk and fermented soy milk have protective effect on NAFLD rat liver.