Study On Immune Milk With 9 Milk Antibodies Against Pathogenic Bacteria Of Enterobacteriaceae

Objective: Selecting 3 strains pathogenic E.coli, 3 strains Salmonella and 3 strains Shigella as antigens to make the mixed vaccine, and gestation cows were immunized systemically. We can prepare immune milk with 9 milk antibodies. Methods: Experimental cows were immunized systemically three times before parturition and after parturition they were immunized systemically three times again. After parturition we collected the milk and researched the change of the total IgG and specific IgG in the milk. We also researched the change of the total IgG after the heat treatment and acidity treatment. Results: The antibody titers against 9 pathogenic bacteria in immune colostrums were 2¹⁰-2¹², 2-9 folds of control regular colostrums. The antibody titers against 9 pathogenic bacteria in immune milk were 2⁶-2¹⁰, 8-14 folds of control regular milk. The denaturation percentages of IgG after treatment at 60℃ 10min, 60 ℃ 30min, 70 ℃ 10min, 70 ℃ 30min, 80 ℃ 10min, 80 ℃ 30min were 14.81%,25.20%,76.44%,85.58,100%,100%, respectively. The retain percentages of IgG after treatment at pH2 10min, pH2 30min, pH4.5 60min, pH4.5 120min were 66.69%,56.55%,100%,100%, respectively. Conclusion: Immunized systemically could not increase the concentration of total IgG in the immune milk ,but significantly heighten the specificity IgG of immune milk .