Effects Of Uygur Medicine Xipayi Kuijie'an On Gene Expression Profile Of Rats With Ulcerative Colitis

Objective: Using DNA microarray to analyze the gene expression spectrum of rats with ulcerative colitis (UC) and we gets the following finding. To screen differential expression genes and to investigate aberrant gene expression of UC in rats. Using DNA microarray to explore the pathogenic mechanism of UC and to analyze Uygur medicine Xipayi Kuijie′an (KJA) pharmaco mechanism. Methods: The rats randomly divided into: normal group and model group 2, 4-dinitrochlorobenzene (DNCB) and acetic acid are used to copy right UC in Wistar rats. Then the rats randomly divided into: normal saline (NS) treated group as negative control, Xipayi KuiJie'an interfered group (large-dosage group, middle-dosage group and small-dosage group). The whole condition were observed in all groups after 20 days treatment. This article using the technology of gene chip, to analysis the gene genealogy in rat of UC by Jingxin 27K Rat Genome Array, then want to look for the differences in rat genes. Real time RT PCR analysis were used to confirm the results of the microarray. Results: The symptoms, physical signs of UC rats were closely correspondent to the UC indexes. Pathological section of colon showed that there were ulcerations in model group. Gene expression showed that as compared with the normal group, in the model group, Screening showed 1054 differentially expressed genes. of which, there were 667 up-regulated expression genes, Among these genes, 414 genes were down-regulated between the Xipayi KuiJie'an interfered group and normal saline (NS) treated group as negative control; there were 387 down-regulated expression genes, Among these genes, 168 genes were up-regulated expression between the Xipayi KuiJie'an interfered group and normal saline (NS) treated group as negative control. The results of microarray were analyzed by pathway: most of them were were related with cell signaling, cell transport, cytokine, cell transcription regulation, cell receptors, cell metabolism, and immune function, inflammation as well. Several interesting genes were identified after pathway analysis, namely, TLR2,TLR6,NF-κB,IL-1β,CCL3 and TGF-β1. The real-time quantitative PCR analysis revealed a similar expression pattern to microarray results. Conchision: Rat UC model was successfully developed by using 2, 4-dinitrochlorobenzene (DNCB) and acetic acid. KJA has a good therapeutic effect on ulcerative colitis in rats. Uygur medicine KJA can cause the remarkable changes of the gene expression profiles in rat with ulcerative colitis. The expression of the differentially genes in colon tissues may be associated with the occurrence of UC. Further analysis of the differentially expressed genes might help reveal the pharmaco mechanism of Uygur medicine KJA medicinals, and provide the insights into the molecular mechanism of the process of ulcerative colitis.