| OBJECT:To study the expression and putative role of uncoupling protein 2(UCP2) in rat kidney after ischemic reperfusion (I/R)injury.METHODS:A total of 20 Sprague-Dawley rats were randomized into two subgroups:1)sham group(n=10); 2) I/R(n=10). peritoneally for 7 days before I/R injury. I/R injury was induced by clamping both renal arteries for 45 min, and the rats were killed 24 h later. Sham operations were performed in a similar manner, except that the renal vessels were not clamped. Both blood samples and tissue specimens were collected. The concentration of MAD, SOD, and GSH-Px was evaluated by ELISA, and the expression of UCP2 mRNA was studied by RT-PCR.RESULTS:Compared with the sham-treated rats, I/R rats showed an increase in serum MDA (12.14±2.35nmoL/mL vs.5.86±0.53 nmoL/mL, P<0.01), a decline in the level of renal SOD(29.42±9.41 U/mL vs.15.11±3.79U/mL, P<0.01) and GSH-Px(53.06±4.68 Uvs.40.98±4.21U, P<0.01).RT-PCR revealed that the expression of UCP2 mRNA expression was significantly increased when compared to the sham group (P<0.01).CONCLUSIONS:The expression of UCP2 mRNA significantly increased in rat kidney in response to ischemia reperfusion injury, and that this may augment the ability against oxidative stress during ischemic-reperfusion.KEYWORD:UCP kidney ischemic-reperfusion oxidative stress... |
