The Effect Of Spinal Cord Injury Rats Nerves Function And 5-ht In One Of The Function By Treadmill Training

Objective:Observation spinal cord injuries after the rat on the limb of the function of training, sports scores (BBB), sports cause potential and spinal cord injury in the present quality of 5-HT, explore training in 5-HT in treadmill training for spinal cord injury rats nerves function return mechanism.Materials and Methods:SD 30 adult female rats, weighing 220-250g, NYU Preparation of spinal cord injury were randomly divided into 2 groups: experimental group and control group. The experimental group started walking a week after intervention began speed 5m/min, for 15 minutes, after increasing every week, training a total of 8 weeks,5 times a week. Control group without any intervention. During the week of the two groups BBB score records,8 weeks after the rats were exercise evoked potential and 5-HT immunofluorescence staining.Result:1. Treadmill training 2-8 week, experimental group at the same time the grading BBB more have increased, and the 2,4,5,6,7,8 weeks groups have a significant gender difference is the second week, and training for the gradingBBB (2.63±2.4) points, and experimental group for (6.39±2.88), p <0.05. Training four to eight weeks, and experimental group is typed groupbbb a marked increase in grade ratings, and experimental group has become the trend of stable,8 weeks for the group test typed(10.10±2.12), experimental group for (13.39±2.07), p<0.01 and training to improve the spinal cord injury on a large limb of the functions of the movement.2. Experimental rats evoked potential latency was significantly shorter than the control group compared with the experimental group incubation period (4.80±2.28) ms, in the control group (5.20±0.56) ms, p<0.05; amplitude increased significantly in amplitude as the experimental group (0.97±0.21) uV, the control group (0.48±0.18) uV, p<0.05, experimental group latency and amplitude compared with the control group, the data were statistically significant, suggesting that treadmill training can help to improve neurological function in rats with spinal cord injury recovery3. Induced an bbb relevant analysis and test showed that the sports cause an incubation period and BBB grading of a negative, the correlation coefficient r=-0.50, r as in the inspection (p<0.01); volatility and BBB mark is in, the correlation coefficient of the r as the r=0.866 inspection(p<0.01). prompt spinal cord injury rats in the function of a large limb of a function of the motion and confirmed to the large mole on the training function of nerves.4.5-HT immune fluorescent chemical dyeing experimental results show that motion and positive group5-HT of nerves with the original amount increased considerably, typed group (14.0±2.04, a slice) experimental group(32.5±6.12, a slice) (p<0.05) that training can promote spinal cord on the nerves of the plasticity and its a 5-HT of expression.5. Experimental group after the amputation exercise the functions of rats mark (BBB) and spinal cord in 5-HT dependency analysis showed that the expression of 5-HT and BBB functions related to sports scores, is a correlation coefficient r=0.925(p<0.05) that training can walk through the promotion of 5-HT increasing number of amputation and improve the rats and exercise the functions.6. Sports cause potential and spinal cord in 5-HT expression of the relevance of the analysis showed that experimental group sports cause potential of 5-HT incubation period with the correlation coefficient R=-0.765(p<0.05);the sports cause potential and 5-HT expression to their;sports cause potential of volatility and 5-HT the correlation coefficient r=0.892, as the r inspection (p< 0.01) that the motion and cause an expression of 5-HT is in the relevant; 5-HT expression of the large mole is a function of conduction.conclusion:1. to promote training treadmill spinal cord injury rats nerves.2. function of training treadmill can promote the middle of the spinal cord was the plasticity and 5-HT expression.