Expression And Significance Of S100 Family In Pancreatic Cancer

Objective To observe the expression differences of S100A4, S100A6, S100P mRNA in human pancreatic carcinoma, and to explore the distributions of S100 subtypes and the relationship between the subtypes and the genesis and development of pancreatic carcinoma. Methods First, the differential expression of four S100 genes was examined by RT-PCR in pancreatic carcinoma tissue and normal pancreatic tissue. All results were analyzed with SPSS10.0 forWindows.Then the S100P gene was selected and analyzed by the Western bloting hybridization in pancreatic cancer. Results S100A4, S100A6, and S100P mRNA in pancreatic carcinoma were highly expressed as compared with normal pancreatic tissue (P<0.01). The expression level of S100A4 mRNA was significantly correlated with the differentiation degree of pancreatic carcinoma, invasion and metastasis(P<0.01) .The S100P protein expression in pancreatic cancer tissue, cell lines, blood serum. There was not statistically significant difference percentage of positive case between the S100P and CA199, CEA. Conclusion quantitation of mRNA of S100A4, S100A6 and S100P is a promising tool for diagnosis of pancreatic cancer, and that they may be a promising therapeutic target for pancreatic cancer. The detection of S100A4 is one of clinical value in the evaluation of malignant degree and prognosis of it.