Expression And Clinical Significance Of Pdgf-d In Bladder Cancer

Objective: To investigate the expression of PDGF-D in bladder transitional cell carcinoma (BTCC) , and its clinical significance in tumorigenesis and progression of BTCC.Methods: By using real-time reverse transcription-polymerase chain reaction (real- time RT-PCR) and Western blotting, the expression levels of PDGF-D mRNA and protein were detected in 62 cases of BTCC tissues, and 10 cases of the normal bladder tissues which were closely adjacent to the carcinoma. The relationship between PDGF-D expression and the clinicopathological features was analyzed.Results: The average expression level of PDGF-D mRNA in BTCC was 1.89 times as much as that in the normal control group. PDGF-D protein expressed in tumor tissues was significantly higher than that in normal bladder tissues (absorbance ratio in the normal bladder tissues was 0.231±0.041, and that in the tumor tissues was 0.689±0.083, P<0.05). Moreover, with the grades going up and appearance of lymph node metastasis, the expression levels of PDGF-D mRNA and protein were increased gradually (P<0.05). But in different clinical stages, the expression levels of PDGF-D mRNA and protein had no significant difference (P>0.05).Conclusion: The overexpression of PDGF-D is closely correlated with the occurrence, differentiation and lymphatic metastasis of BTCC, and play an important role in carcinogenesis and progression of bladder cancer. To investigate the exact mechanisms of PDGF-D overexpression and the corresponding PDGF-D signaling in bladder cancer, may provide new ideas and methods for exploring specific and effective pharmacological therapy of bladder cancer.