Effects Of Low Doses Levodopa Administration On Striatum Acetylcholine And M1, M2 Receptors Expression In Parkinsonian Rats

【Objective】To investigate the effects of low doses of levodopa administration on striatum acetylcholine Ml,M2 receptors and acetylcholine content expression in Parkinsonian rats .【Methods】6-OHDA was stereotaxieally injected into rats to make rats model of P D; Rat s were randomly divided into 5 groups , the blank group, The model group,the drug7d,14d,21d group,Immunohistochemistry was measured by Ml and M2 receptors in the striatum expression. HPLC-ECD was used to measure the content of Ach in the striatum at different survival times 7d,14d,21d.【Results】Ml receptors in the expression of a rat model of Parkinson's disease the average optical density is higher than the normal control group; after 14d the average optical density of Ml receptor protein decreased significantly compared with model group (P<0.05), the medication 21d Ml receptor expression in post-recovery; M2 receptor protein average optical density in Parkinson's disease model group than in the control group decreased significantly(P<0.05),but the drug had no significant pick-up after the intervention(P<0.05).The level of Ach decreases significantly in the PD rats, 7d intervention with levodopa, the concentration of acetylcholine was significantly lower than the model (P <0.05), but the drug 14d, 21d after the acetylcholine content increased when compared with the model group (P<0.05).【Conclusion】PD rats with low doses of levodopa after the intervention of the M1 receptor protein expression in the striatum and acetylcholine content is a dynamic process of change. M2 receptors in the drug show no significant change after the intervention.