The Change Of Cholinergic Nervous System In The Striatum Of PD Rat

Partâ… :The level of DA and Ach in the striatum of PD ratObjective : To observe the change of Dopamine ( DA) and Acetylcholine(Ach)in the striatum of Parkinson's disease (PD) rat. Methods: 6-OHDA was stereotaxically infused into rats to make rats model of PD. HPLC was used to measure the level of Ach and DA in the striatum at different survival times(1w, 2w, 3w ) and of L-dopa-treated group. Results : The level of Ach in the affected part did not change after one week of the operation, and increased gradually in the following two weeks; the level of DA decreased obviously(P<0.05), L-dopa administration produced a bit of decline in Ach , the level of DA resumed as compared with before. Conclusions : The level of Ach increased and DA decreased obviously in the PD rats. L-dopa can lessen the change of Ach and DA.Partâ…¡:Expression of acetyl cholinesterase and M1 acetylcholine receptor in the striatum of PD ratObjective : To observe the change of Expression of acetyl cholinesterase and M1-acetylcholine receptor in the striatum of Parkinson's disease rat. Methods : 6-OHDA was stereotaxically infused into rats to make rats model of PD. The change of Expression of Ach E and M1-acetylcholine receptor in the striatum was observed by using immunohistochemical staining at different survival times(1w, 2w, 3w ). Results : Ach E positive cells increased at 1w, but obviously decreased at 2W and 3W(P<0.05);M1-acetylcholine receptor positive cells do not change at 1w, but obviously increased at 2W and 3W(P<0.05).The expression of Ach E and M1-acetylcholine receptor resumed after L-dopa administration. Conclusions : The expression of Ach E receded and the expression of M1-acetylcholine receptor increased in the striatum of PD rats . L-dopa can lessen the change.