Effects Of Xiaogutong Decoction On The Expression Of Tgfβ1 And Bmp2 In Rat Osteoarthritis

ObjectiveTo analyze the effects on the expressions of transforming growth factorβ1(TGFβ1) and bone morphogenetic protein2(BMP2) of knee cartilage,membran and serum in rat osteoarthritis after treatment by the traditional chinese medcine(TCM)Xiaogutong decoction. To discuss the mechanism of TCM,and find a better method to solve a series of clinical disorder and improve patients'life quality.MethodsForty SD rats were randomly divided into four groups(n=10), and each animal except normal groups was treated. The osteoarthritis models were made by injecting papain into the knee joints. Then the rats were begin to lavage. The normal and model group were given 0.9% sodium chloride, TCM group were given Xiaogutong decoction fluid, western medcine group were given diclofenac sodium.They were lavaged for eight weeks.Then all rats were killed and took the specimen of cartilage,membran and serum. HE staining specimens were observed by microscope, and the expressions of TGFβ1 and BMP2 were detected by streptavidin—peroxidase immunohistochemical method. The protein content of TGFβ1 and BMP2 in serum were detected by ELISA.ResultsThe immunohistochemical result found that TGFβ1 and BMP2 proteins were express in articular cartilage and synovium in every groups. There were obviously different on the expressions of the TGFβ1 and BMP2 in cartilage and synovium. The expressions of the TGFβ1 and BMP2 in cartilage of TCM group were higher than model and western medcine group(P<0.01). There were no statistical significance between TCM and normal groups(P>0.05). The protein content of TGFβ1 and BMP2 in serum had similar tendency to articular cartilage. The expressions of TGFβ1 and BMP2 in synovium of TCM group were lower than model and western medcine groups(P<0.01). There were statistical significance between TCM group and western medcine groups(P<0.05). There were no statistical significance between TCM and normal groups(P>0.05).ConclusionXiaogutong decoction can up-regulate the expressions of the TGFβ1 and BMP2 in cartilage and content in serum of OA . It can delay cartilage degradation, and has effective in cartilage protection and repairing. The expressions of the TGFβ1 and BMP2 in synovium are tend to decline, Xiaogutong decoction perhaps may reduce the fibrosis in synovium, and decrease the cell differentiation, it also may reduce the synovium inflammation to some degree.