The Clinical Research Of The Effects Of Oral Administration Of Probiotics To The Intestinal Tract Immunity Of Preterm Infants

Objective To investigate the effects of oraladministration of probiotics to the intestinal tract immunity of preterm infants by observing their digestive system clinical situation and detecting some immunization indicators of them.Methods Selecting four hospitals as the experimental co-hospital.Designing the table for clinical observation of preterm infants.Selecting preterm infants who meet the selected conditions as the subjects.Dividing them into the experimental group or the control group randomly as soon as they were hospitalized.Feeding the experimental group with the Bifid triple viable capsule (Bifidobacterium, Lactobacillus acidophilus, Enterococcus triple viable ),but warm water for the control group,the experiment lasts 21days.Observing their digestive system clinical situation.Collecting their blood samples and stool specimens at the 1,7,14,21 day for detecting as follow: IL-2,IFN-γin serum and secretory IgA in fecal contents by ELISA, CD4+ , CD8+ T lymphocytes and thier ratio by Flow Cytometry, lactos content in stool by improved Benedict's Reagent.By comparing their variability to discuss the effect of oraladministration of probiotics to the intestinal tract immunity of preterm infants.Result1. There were 115 preterm infants who finished all of the experiment,54 for the control group,61 for the experimental group.2. The incidence of digestive system of two groups in different weeks: there was no difference between them in the incidence of fever(P>0.05),but differed in the incidence of vomiting, constipation, diarrhea, retention and lactose intolerance(P<0.05),and the incidences of the experimental group were obviously lower than the control group's.3. IL-2 in serum of two groups in different weeks: there was no difference between them at the first day (P>0.05) ,but increacing after the first week's loss,and differed(P<0.05).4. IFN-γin serum of two groups in different weeks: the change of concentration of IFN-γin serum were similar with that of IL-2 in serum, there was difference between them at the same time point(P<0.05).5. Secretory IgA in fecal contents of two groups in different weeks: there was no difference between them at the first day and the seventh day (P>0.05) ,but the experimental group's increaced after that,and differed(P<0.05).6. CD4+ , CD8+ T lymphocytes and thier ratio of two groups in different weeks: there was no difference between them at the first day ,the seventh day and the fourteenth day (P>0.05) ,but differed at the twenty-first day(P<0.05).Both groups'were increased at the twenty-first day ,but the experimental group's were increaced more than the control group's.Conclusion1. The probiotics do not increase the risk of infection to preterm infants, it may be safe to preterm infants to take the probiotics for a long time.2. The probiotics can improve preterm infants'bowel function,reduce the incidence of vomiting,constipation,diarrhea,retention and lactose intolerance,increase the quantity of the secretory IgA in fecal contents.It proved that the probiotics can enhance the intestinal tract immunity of preterm infants.3. The probiotics can promote the level of IL-2 and IFN-γin serum,so it can enhance the cellular immunity of preterm infants indirectly.4. The probiotics can raise the cell population of the CD4+ T lymphocytes in blood of preterm infants, promote the ratio of CD4+ and CD8+ T lymphocytes in blood of them, so it can enhance the cellular immunity of preterm infants directly.