The Study Of Imbalanced Amino Acid Solutions By Enteral Nutrition On Hepatocarcinoma Inhibition And Nutritional Status In Tumor Bearing Mice

Objectives:To study the influence of several amino acid imbalance solutions on hepatocarcinoma inhibition and nutritional status in tumor-bearing mice.Methods:According to formulas of amino acid composition in nutrition solutions respectively, the hepatoma22-bearing mice were divided into seven different groups: group A (amino acid balance solution), group B (arginine-enriched amino acid imbalance solution), group C (valine-depleted amino acid imbalance solution), group D (leusine-enriched amino acid imbalance solution), group E (tyrosine-depleted amino acid imbalance solution), group F (phenylalanine-depleted amino acid imbalance solution), group G (serine-depleted amino acid imbalance solution), group H(no Amino acid), group I (compound amino acid imbalance solution). Total gastric-intestinal nutrition solutions had been used for 12 days in Hepatoma22-bearing mice. Indices of hepatocarcinoma inhibition include tumor volume,tumor weight,tumor weight/carcass body weights,necrotic rate of tumor cell,PCNA and DNA ploidy. Indices of nutrition status include weight lost,transferrin(Trf),prealbumin(PA) and T-cell subgroup.Results:All tumor inhibition indices of group B, C, D, E, F, G, H and I were significantly less than those of group A except necrotic rate of tumor cell and DNA ploidy. Cell life cycle analyses:H, I compare with A group, G/G。stage kyto-percentage all step up, S stage andG+M stage descend (P<0.01). The levels of weight lost were significantly more differences in group C,D,E,F,G than those in group A, B, H, I. The level of Trf in these groups was not found significantly differences. The level of PA was significantly more differences in group B than those in group C, H. The levels of T-cell subgroup in group B, I were significantly differences highly than that in group A.Conclusions:Amino acid balance solution, arginine-enriched amino acid imbalance solution, no Amino acid solution and compound amino acid imbalance solution can reserve nutrition status of tumor-bearing mice relatively, whether other amino acid imbalance solutions have similar disadvantageous influence. Amino acid balance solution can stimulate tumor growth, whether all amino acid imbalance solutions have similar inhibition effects.