| Purpose To investigate the effects of spinal anesthesia with different concentrations of chloroprocaine on the ultrastructure and IECa2 +of the rats's spinal cordMethods 39 male SD rats weighing 250-270g were anesthetized with intraperitoneal 10% chloral hydrate 35mg/100g. A polyurethane microcatheter was inserted into the lumbar subarachnoid space. A 3cm catheter degment was left in the subarachnoid space. The rats were randomized to receive normal saline,2.0%(W/V) or 3.0%(W/V) chloroprocaine 40μl. Six hours after the intrathecal administration, part of the animals(n=5) were decapitated and the segment of the spinal cord were immediately removed for electron microscopic examination. Other rats (n=8, every group) were anesthetized to take the segment for detect the IECa2+Results Electron microscopic examination revealed that in animals which received intrathecal normal saline or 2.0% chloroprocaine the mitochondria and endoplasmic reticulum of the neurons in the spinal cord were intact, while in animals which received intrathecal 3.0% chloroprocaine there was swelling of endoplasmic reticulum and vacuole degeneration in one rat of this group. IECa2+ of the NS and 2% CP group hadn't contrast in statistics, but 3% CP group was higher than other groups.Conclusion Spinal anesthesia with 2.0% chloroprocaine hasn't obvious influence on the ultrastructure of spinal cord and IECa2+ intra-cellular, but with 3.0% may be injurious to the spinal cord... |
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