Influence And Significance Of Narrow-band Ultraviolet B (nb-uvb) Phototherapy On Peripheral Blood Cc Chemokines (mcp-1, Mip-1α) And Ccr1mrna In Patients With Psoriasis

Psoriasis is a chronic inflammatory autoimmune disease. However, the pathogenesis and treatment remains the world's problems. In recent years, the relationship between psoriasis and chemokines (include chemokine receptors) has become popular. In this study, enzyme-linked immunosorbent assay (ELISA) technique was used to detect CC chemokines MCP-1 and MIP-1αvalues in peripheral blood of psoriasis patients before treatment and patients after NB-UVB treatment and healthy controls; Reverse transcription-polymerase chain reaction (RT-PCR) technique was applied to semi-quantitatively analyze CCR1 mRNA expression levels in peripheral blood mononuclear cells (PBMC). In order to investigate whether NB-UVB through regulate the expression of certain CC-type chemokine (or its receptor) to achieve the purpose of anti-psoriasis.Objective: To observe effect of narrow-band ultraviolet B (NB-UVB) phototherapy on peripheral blood CC chemokines (MCP-1, MIP-1α) and CC chemokine receptor 1 (CCR1) in patients with psoriasis. Further clarify whether NB-UVB through influencing the expression of MCP-1 and MIP-1αand CCR1 to achieve the purposes of therapy, provide more theoretical basis for NB-UVB in the treatment of psoriasis.Methods: Serum levels of MCP-1 and MIP-1αwere measured by double-antibody enzyme-linked immunosorbent assay (ELISA) in patients with psoriasis vulgaris before and after the treatment with NB-UVB. CCR1mRNA expression levels in peripheral blood were detected by RT-PCR.Results: Before treatment, MCP-1 and MIP-1αvalues in peripheral blood of patients with psoriasis vulgaris were significantly higher than the normal controls. After 15 times phototherapy, the serum levels of MCP-1 decreased significantly, the difference has statistically significant (P <0.01). While the serum levels of MIP-1αshowed no significant difference before the treatment than after the treatment (P> 0.05). CCR1 levels in peripheral blood mononuclear cells of psoriasis patients before treatment was significantly higher than those in the normal controls. After 15 times phototherapy, CCR1 expression levels in peripheral blood mononuclear cells decreased significantly, the difference has statistically significant (P <0.01).Conclusion: NB-UVB partly through reducing the expression of MCP-1 and CCR1 in peripheral blood of psoriasis to achieve treatment objectives.