The Effects Of Thp To The Grow Of Nasopharyngeal Carcinomacell Line Cne2 And The Expression Of Xiap

Objective XIAP (X-linked inhibitor of apoptosis protein) have high expression in many tumors, but it have no expression or low expression in normal tissue. They have a very close relationship between XIAP and tumor occurrence and development. To evaluate the effects of THP (tetrahydnopy- rany adriamyrin, THP) on the proliferation of Nasopharyngeal Carcinoma cell line CNE2 and the expression of XIAP (X-linked inhibitor of apoptosis protein, XIAP) gene. For the clinical treatment of nasopharyngeal carcinoma provide a better method.Methods The Nasopharyngeal Carcinoma cell line CNE2 was Cultured with DMEM medium at 37℃in 5% carbon dioxide incubator. The cell viability of CNE2 cells were assayed with MTT test and Colony forming assay after exposing to a different concentration of THP and the expression of XIAP mRNA detected by RT-PCR assay. Immunohistochemical method test the expossion of anti-apoptosis protein XIAP using cell climbing sheet.Date were analyzed with ANONA and paired T test using SPSS11.0 statistical software.A level of P﹤0.05 was considered statistically significant.Results MTT resulted showed that the suppression ratio of one time of clinical doses was 74±0.3% in 24 hours, the inhibition ratio of one-tenth of clinical doses was 39±0.5% in 24 hours, the inhibition ratio of one percent of clinical doses was 13±0.2% in 24 hours. The suppression ratio of one time of clinical doses was 83±0.4% in 48 hours, the inhibition ratio of one-tenth of clinical doses was 77±1.1% in 48 hours, the inhibition ratio of one percent of clinical doses was 19±0.5% in 48 hours.The effects of THP is of time-effect and dose-effect relationship, the increase of inhibitive effects is companied by increasing of action time and concentration of THP. Colony forming assay resulted showed the clone numbers of one time of clinical doses(90±5) and control group(403±10)were different. The clone numbers of one-tenth of clinical doses(264±8) and control group were different. The more increase drug concentration, the more the clone numbers were on the decrease(P﹤0.05).The PT-PCR resulted showed that the expression of XIAP mRNA decreased .The ratio of XIAP andβ-actin were 0.97±0.015 on control group. The ratio of XIAP andβ-actin were 0.53±0.020 on one-tenth of clinical doses. The ratio of XIAP andβ-actin were 0.18±0.011on one time of clinical doses.There are have statistically significant(P﹤0.05). Immunocytochemical method show that anti-apoptosis protein XIAP was mainly expressed in cytoplasm and the nucleus .In drug groups the drug concentration increased, decrease of XIAP protein more compared with control group.Conclusions1. The THP inhibits cell proliferation of CNE2 and cell line has a sensitivity to this drug.2. The THP reduce expression of the XIAPmRNA and XIAP protein of CNE2.3. The drug concentration increased, apoptosis more. It may be due to decrease expression of XIAP.4. Detection of XIAP will be helpful for diagnosis and clinical staging of NPC.It deserves our further study.