Effect Of Dhyz On Oxidative Stress And Calcium Ion Channel In Pc12 Inducedby Ageing Treatment Of Aβ_25-35

Objective: This study use chemical,biochemical and other advanced means and methods of detection to explore viability,the cell structure changes and the regulation of apoptosis of PC12 cells injured by Amyloidβ-protein and affected by the cerebrospinal fluid-Amyloid with DHYZ.Aβ25-35 PC12 cell injury model,the current international standards of AD model,was established and the neurotoxicity effect of apoptosis indeed by Aβ25-35 was confirmed.Methods: The cultured cells of PC12 grown in the log phase were divided into 6 groups: normal control group,model control group,Aricept control group,low dose of DHYZ group,medium dose of DHYZ group and high dose of DHYZ group.The above 6 groups were respectively added 10% normal cultured fluid,10% normal cerebrospinal fluid,10% Aricept cerebrospinal fluid,5% DHYZ cerebrospinal fluid,10% and 20% dose DHYZ cerebrospinal fluid,in sequence which was then added cultured fluid to the equal standary.The abstained examples were hatched for 2 hours under 37℃.The same dose of cultured fluid were added to the normal group.The aged Aβ25-35 were added to the other five groups with the final concentration of 10 umol/L.All the whole six groups were hatched for 24 hours under 37℃.After centrifuging,the vitality of the cultured cells were measured with the methods of MTT and LDH. And detected the MDA, SOD, CAT,GSH-Px levels;also the intensity of calcium combined with fluorescence probe.Result: The results showed: 1.DHYZ can enhance MTT metabolism rate, reduce the release of LDH.It can improve the cells survival activity after injured by Aβ25-35. 2.DHYZ could increase the SOD,CAT,GSH-Px levels and decrease the MDA levels in the cell culture fluid of PC12, increase the scavenging capacity of free radical.3.DHYZ can inhibit calcium overload, protect cell membrane structure. Conclusion: DHYZ may inhibit calcium overload, reduce LDH release and enhance antioxidant enzyme activity of PC12 cells,which were injuried by aged Aβ25-35 ,DHYZ can improved cells state, increase free radical, clear the free radical to protect the cell vitality.