The Research Of Rubus Parvifolius Extract On Anti-hepatitis B Virus In Vivo And In Vitro

Objective To observe the role of Rubus parvifolius extract(extract of Ethyl acetate)on against duck hepatitis B virus in vitro and in vivo.Methods In vitro experimental,the HepG 2.2.15 cells with exponential growth were divided into 7 groups, blank control group: 10% FBS in RPMI 1640 medium; Rubus parvifolius extract Group: 400,200,100,50 mg / L; positive control group: lamivudine (10^-6mol / L); vehicle control group: 1% DMSO. Each concentration of complex is located three holes, Every hole is 20μL, 37℃, 5% CO₂ ,they were measured after 72,96 hours . To determinate expression of HBsAg and HBeAg in the supernatant culture medium by ELISA. In vivo experimental, PCR technology Screening were used to selete three-day-old positive D-HBV 50 ducks . The duck were randomly divided into blank control group and Rubus parvifolius extract with concentration of high, medium and low-dose group and lamivudine group. Before the medication,The medication of 7,14 and 21 days ,and 3 days and 7 days after withdrawal, take each group duck blood for duck serum DNA dot blot hybridization, computing duck serum DHBV-DNA density,the last time take the pathological liver tissue to do the Histological observation.Results Each dose group of Rubus parvifolius extract were inhibited the expression of HBsAg and HBeAg and have been shown a concentration-dependent and time-dependent, It has suggested that Rubus parvifolius extract significantly inhibits the expression of HBsAg and HBeAg. In this experiment, the virus control animals stable levels of serum DHBV-DNA positive drug lamivudine (3TC) oral 100mg/kg/d×21d can significantly inhibit the serum DHBV-DNA(compared with blank control group P<0.05), 3 days and 7 days after drug withdrawal wound produce a significant rebound in DHBV-DNA(compared with blank control group P<0.05). After 21 days of Subjects drug Rubus parvifolius extract treatment ,with various doses of serum DHBV-DNA at each time point does not show a significant difference(compared with blank control group P>0.05). The control group at each time point changes of serum DHBV-DNA was no difference(compared with blank control group P>0.05). It indicates that Rubus parvifolius extract of each dose group does not inhibitory effect on DHBV-DNA in the duck. among the groups, Pathological examination of liver ,and has no significant difference(compared with blank control group P>0.05).Conclusion Rubus parvifolius extract main function of anti-hepatitis B virus play a role through its prototype. the prototype through the metabolism of the body in the duck, The role of anti-hepatitis B virus was lost.