Dioscorea Nipponica And Dioscorea Rapid Propagation Techniques

Dioscoreaceae plants are important economic plants. Diosgenin is the main active ingredient of Yams which is an important raw material for synthetic steroid hormone. On the basis of its high medical value Yam plants are in great demand. Wild Yam plant resources are getting less and less and can not meet the need of production. They are all artificial cultivation currently. Diosgenin exist in sect stenophora Yam plants. There are two kinds of Yam plants in Mexico called D.composite Hemsl. and D. spiculiflora Hemsl which contain 13% doisgenin. There is one kind of Yam plant in Hubei which contain 16.15% doisgenin. This Yam plant own the higtest content in the world[64]There are many problems when cultivate Dioscorea zingiberensis, such as less variety, Slow reproduction. Cultivation can not meet the demand of production. Dioscorea composita Hemsl which content more Diosgenine can not bear the climate of winter in North of China. In order to solve the above two problems (slow reproduction and climate adaption), we choose Dioscorea Zingiberensis CH Wright and Dioscorea composite Hemsl for rapid propagation research.We do research on the basis of former study. See blow the main conclusions:1.abduction of axillary budsThe optimization of hormone ratio of Dioscorea Zingiberensis CH Wright is 8 MS+6-BA 1.0 mg/L+NAA 0.2 mg/L, it lead to 94.44% induction rate.The optimization of hormone ratio of Dioscorea composite Hemsl is MS+1.0mg/L 6-BA+ 0.1mg/L NAA, it leads to the highest induction rate.2. Rooting inductionRooting culture hormone ratio is 7 1/2MS+NAA 1.0mg/L, lead to 80% rooting rate.Rooting culture hormone ratio is 1/2MS+6-BA0.2mg/L+NAA0.5mg/L+KT0.5mg/L, leads to the highest rooting rate; small activated carbon can prevent browning and increase rooting rate to 89.73%.3. TransplantationThe survival rates of test-tube plantlets will up to 98% when we use the following methods:Transplantation medium= (pearlite:vermiculite:humus=1:1:1) with little 1/2 MS liquid media and 0.1% derosal.Using above methods and conclusions we can produce more than 10 plants by one maternal plant. It is give us great help for rapid propagation of these two kinds of Yam plants.The study offers us a whole refined process for rapid propagation of these two kinds of Yam plants and gives effective supports for the further study.