Ultrasonic Assisted Extraction Honeysuckle Flavonoids

Flos Lonicerae Japonicae are the drying buds of Lonicera japonica Thunb,a perennial evergreen winding shub of Caprifoliaceae.Flos Lonicerae Japonicae is used in Chinese herbal medicine for latent-heat-clearing and detoxicant.With the exception of luteoloside,Flos Lonicerae Japonicae also contains many kinds of flavones including rutin,quercetin,luteolin, luteolin-7-glucoside.Modern medical studies have shown that flavonoids have a reduced myocardial oxygen consumption to coronary and cerebral vascular flow to increase,anti-rhythm,soften blood vessels,lowering blood glucose,blood lipids and so on.At present,the Lonicera japonica Thunb flavonoids extraction methods commonly used are:organic solvent extraction,water solvent extraction and so on.These methods have some shortcomings,such as low extraction efficiency, solvent consumed in large quantities.Thus,how to enhance the efficiency of ultrasonic extraction is a hot topic of this field in recent years.Ultrasound-assisted extraction technology as an efficient extraction of energy-saving technology,has a good application prospects in the active ingredient of plant.In this experiment ultrasound-assisted extraction technology is used in Lonicera japonica Thunb flavonoids extraction research,and with the traditional hot water extraction method to compare.Through the response surface experimental design to explore the best conditions of ultrasound-assisted extraction of Lonicera japonica Thunb flavonoids and to establish the polynomial quadaratic equation model.This provide an important basis for the further experimental studies and more rational use of valuable Lonicera japonica Thunb resources.In the hot water extraction method,the optimal process is ascertained by the single factor experiment and the L₉(3⁴) orthogonal design.The optimal extraction conditions are that the extractive time is 2.5h,the extractive temperature is 80℃,the ratio of water to material is 45:1. Under above-mentioned conditions,the experiment is repeated for 3 times and the average extraction efficiency of the Lonicera japonica Thunb flavonoids is 8.01%.The optimal process on the ultrasound-assisted extraction method is ascertained by single factor experiment and L₉(3⁴) orthogonal design.The optimal extraction conditions are that the ultrasonic extraction time is 20min,the ultrasonic power is 400w,and the ratio of water to material is 35:1. Under above-mentioned conditions,the experiment is repeated for 3 parallel times and the average extraction yield for the Lonicera japonica Thunb flavonoids is 8.33%.In order to study the extractive process for the ultrasound-assisted extraction method deeply,the extraction for the Lonicera japonica Thunb flavonoids is discussed by Box-Behnken design.Three main factors affecting total flavonoids extraction yield are the ultrasonic extraction time,the ultrasonic power,the ratio of water to material, with these factors,it is carried on for Box-Behnken response surface design for the three factors and three levels.Also,the predictive model of polynomial quadratic equation is analysed by Design Expert software.They are discussed for the key factors affecting ultrasonic extraction and their interactions by the response surface and contour of the model,the results indicate that the optimum extraction condictions of the total flavonoids are as follows:ultrasonic extraction time 21.35min, ultrasonic power 472.24w,ratio of water to material 34.62ml/g.For practical convenience,the extraction efficiency for the flos lonicerae japonicae flavonoids is 8.87%,after the condition is amended appropriately.The research shows that ultrasound-assisted extraction method has higher efficiencies than the hot water extraction method.This is mainly reflected in:short ultrasound-assisted extraction time, low operation temperature,less solvent consumption,higher extraction efficiency and more convenient to operate.Based on the single factor test,we have established the polynomial quadaratic equation model of ultrasound-assisted extraction of Lonicera japonica Thunb flavonoids through the response surface experimental design.The optimized extraction conditions are as follows:ultrasonic extraction time 21.35 minutes,ultrasonic power 472.24w,ratio of water to material 34.62ml/g. Under the optimized conditions,the extraction rate of the total flavonoids is 8.87%.The modle fitted can be able to better meet the actual.This is an important theoretical significance and application value for further study of the extraction of Lonicera japonica Thunb flavonoids.