Kwai Resistance To Tmv Extraction And Separation Of Active Ingredients And Its Preparation

Tobacco mosaic virus (TMV) is one of the important diseases in agriculture productions, almost all of agronomic crops are endangered by TMV. It is difficult to control, because of its absolute parasitism. At present, the profitable antiviral medicament products are very rare in both here and abroad. With the development of the living standard, people have paid more and more attention to the environmental problem. Botanical pesticides are a better choice in the future agriculture, for its characteristics such as low toxicity, low residual, highly activity and environment compatibility. Through tracking biological activity, the active component of anti-TMV was gained from of Semiaquilegia adoxoides (DC) Makino (a kind of herbal medicine) earthnut by extraction and separation. It will maybe provide the important academic theory and practical direction for screening active components of antiviral agents.On the base of the early studies, the anti-TMV active component TK and effective component TK-1 were screened from S. adoxoides extract by half-leaf dry spot method. The series of preparations of the natural product No. 2 emulsion oil in water (EW) against TMV was also made through formulation processing and detecting. The results are as follows:1.In this study, the chemical composition of S. adoxoides was tested extensively. The results show that the S. adoxoides extract includes sugars, organic acids, peter anthracene, phenols and volatile oil.2. In this study, biologic activity of S. adoxoides extracts against TMV was determined by adopting half-leaf dry spot method, the experiment operating condition of half-leaf dry spot method thus was established. The results of biologic activity show that biologic activity of S. adoxoides extracts against TMV varied with treatment time. The effect of inhibiting multiplication means that TMV invaded tobacco leaf within 2h; after 2h, TMV had invaded and propagated cosmically; the best treatment time was 1h after inoculation; S. adoxoides extract mixed for 30 min with the same volume of the TMV had the best effect. 3. In this study, the method of separating active ingredients from S. adoxoides was improved. The powders of S. adoxoides root were extracted and then purified by using TLC combined with column chromatographic analysis. The effective components B4-3 was gained by using chloroform-methanol (83: 17) to elute gradiently chloroform extract (B), and its protection effect and treatment effect was 52.54 percent and 48.69 percent, respectively. The active ingredients C5-5 was also gained by using chloroform-methanol (75: 25) to elute gradiently ethyl acetate extract (C), and protection effect and treatment effect of C5-5 was 42.98 % and 62.07 %, respectively.4. In this study, the series of preparations of TK-1 EW against TMV were formulated. Prescription No. 2 EW and its quality control targets were confirmed primarily. This EW is consisted of effective component TK-1, solvent dimethylsulfoxide, emulsifier OX-7513, dispersant glycol, antifreeze cymene-fibrinuria, and additives such as pH regulator, thickener, coloring agent and odor-conditioning agent. After detection of the formulation processing and the quality, each quality control targets were accord with requests.5. Biologic activity of No. 2 EW against TMV in tobacco was studied. The results show that, in vivid, protection, treatment and inhibition rate in heart shape tobacco (Nicotiana glutinosa) was 62.05%, 64.28%, and 68.64%, respectively; control effect in common tobacco K326 (Nicotiana Tableacum var) was 68.64%. ED₅₀ of No. 2 EW was 724.44 mg/L, the regression equation was y = 1.0501x+2 (r = 0.9874).